一种获得针对偶联至牛γ球蛋白的二硝基苯基的体外初次免疫应答的简单方法。
A simple method to obtain an in vitro primary immune response to dinitrophenyl coupled to bovine gamma-globulin.
作者信息
Del Guercio P, Brugère S
出版信息
Ann Immunol (Paris). 1980 Sep-Oct;131D(2):153-9.
PMID:6162415
Abstract
This paper describes a simple method of achieving a primary in vitro response to dinitrophenyl coupled to a soluble protein. Mice are carrier-immunized, then irradiated and restored with spleen cells from normal donors. Spleen cells are then cultured in the presence of dinitrophenyl on homologous carrier. The anti-hapten antibody synthesis is dose-dependent, and the highest response is obtained with the lowest dose used in the experiments. The higher level of hapten conjugation reduces the immune response.
摘要
本文描述了一种实现对与可溶性蛋白质偶联的二硝基苯基产生初次体外反应的简单方法。用载体免疫小鼠，然后进行照射并用正常供体的脾细胞进行恢复。随后在同源载体上二硝基苯基存在的情况下培养脾细胞。抗半抗原抗体的合成呈剂量依赖性，在实验中使用的最低剂量时获得最高反应。较高水平的半抗原偶联会降低免疫反应。
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A simple method to obtain an in vitro primary immune response to dinitrophenyl coupled to bovine gamma-globulin.一种获得针对偶联至牛γ球蛋白的二硝基苯基的体外初次免疫应答的简单方法。
Ann Immunol (Paris). 1980 Sep-Oct;131D(2):153-9.
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