Bistoni F, Mosci L, Vecchiarelli A, Marconi P, Pitzurra M
Ann Sclavo. 1977 Sep-Oct;19(5):1006-12.
Haemagglutinating antibodies have been assessed in rabbits undergoing active- passive immunization against tetanus. The animals received 6 injections of horse immune serum, 400 UI/kg, and A1PO4 adsorbed toxoid, 0.35 Lf/kg, every 30th day. One the 5th day, after the first injection, E.A. antibodies appeared, at low levels, as a result of a passive immunization. Thereafter the tests became negative, up to the 70th day, when an active immunization emerged, with a 25 days delay in comparison with controls. Neutralization test in vivo behaved in the same way. The results stress once more the need to give up the use of heterologous immune sera in tetanus prophylaxis, in active-passive immunization as well. Arguments adding force to this point of view are: the sensibilization against heterologous proteins, the very low (if any) passive protective action, and, last not least, the delay in the emergence of active immunization: the only reliable shield against tetanus.
已在接受破伤风主动-被动免疫的兔子中评估了血凝抗体。这些动物每隔30天接受6次注射，每次注射400国际单位/千克的马免疫血清和0.35絮状单位/千克的磷酸铝吸附类毒素。首次注射后第5天，由于被动免疫，出现了低水平的E.A.抗体。此后，检测结果一直呈阴性，直到第70天出现主动免疫，与对照组相比延迟了25天。体内中和试验也表现出相同的情况。结果再次强调，在破伤风预防中，无论是主动-被动免疫，都需要放弃使用异种免疫血清。支持这一观点的理由有：对异种蛋白的致敏、极低的（如果有的话）被动保护作用，以及最后但同样重要的是，主动免疫出现的延迟：破伤风唯一可靠的防护。
