Ree H J, Song J Y, Leone L A, Crowley J P, Fanger H
Hum Pathol. 1981 Jan;12(1):49-59. doi: 10.1016/s0046-8177(81)80241-9.
The occurrence and pattern of cytoplasmic muramidase containing histiocytes were studied by the unlabeled antibody peroxidase-antiperoxidase method in biopsy material from patients with Hodgkin's disease, non-Hodgkin's lymphomas, and reactive hyperplasia. The majority of lymph nodes from patients with Hodgkin's disease, nodular lymphoma, and reactive hyperplasia gave positive staining reactions when tested in this manner. Differences in the staining pattern were observed for the different conditions studied. In general, stain positive cells occurred in one of the following four patterns: nodular, dispersed, aggregating without background stain, or aggregating with background stain (mottling pattern). The nodular and aggregating without background stain patterns were not specific and were seen in various conditions. The dispersed pattern, however, was observed only in some cases of non-Hodgkin's diffuse lymphomas, suggesting a subgroup of tumors characterized by active participation of reactive histiocytes. The mottling pattern was virtually limited to Hodgkin's disease. Since the mottling pattern appeared to be produced by virtue of a large amount of extracellular muramidase, the elevation of the serum muramidase level in Hodgkin's disease may be related to enzymatically active secretory histiocytes. Moreover, the mottling staining pattern was observed frequently in the lymphocytic predominance and nodular sclerosis type of Hodgkin's disease, but relatively infrequently in the mixed cellularity or lymphocytic depletion types, suggesting that the variation in histiocytic activity may be related to the course of the disease. The decreased staining reaction observed in the latter two categories could not be accounted for by a decrease in the numbers of histiocytic cells in hematoxylin and eosin stained sections, suggesting that release or synthesis may be defective in those unfavorable types of Hodgkin's disease.
采用未标记抗体过氧化物酶-抗过氧化物酶法，对霍奇金病、非霍奇金淋巴瘤及反应性增生患者活检材料中含胞质 muramidase 的组织细胞的发生情况及模式进行了研究。霍奇金病、结节性淋巴瘤及反应性增生患者的大多数淋巴结经此方法检测均呈阳性染色反应。在所研究的不同情况中观察到了染色模式的差异。一般来说，染色阳性细胞呈现以下四种模式之一：结节状、散在分布、聚集但无背景染色或聚集并有背景染色（斑驳模式）。结节状及聚集但无背景染色的模式并非特异性的，在多种情况下均可见到。然而，散在分布模式仅在某些非霍奇金弥漫性淋巴瘤病例中观察到，提示存在一个以反应性组织细胞积极参与为特征的肿瘤亚组。斑驳模式实际上仅限于霍奇金病。由于斑驳模式似乎是由大量细胞外 muramidase 产生的，霍奇金病患者血清 muramidase 水平的升高可能与具有酶活性的分泌性组织细胞有关。此外，在淋巴细胞为主型和结节硬化型霍奇金病中经常观察到斑驳染色模式，但在混合细胞型或淋巴细胞消减型中相对较少见，这表明组织细胞活性的变化可能与疾病进程有关。在后两类中观察到的染色反应减弱不能用苏木精和伊红染色切片中组织细胞数量的减少来解释，提示在那些预后不良的霍奇金病类型中释放或合成可能存在缺陷。
