Dodon M D, Quash G A
Immunology. 1981 Mar;42(3):401-8.
Native IgG of human or rabbit origin, from which terminal sialic acid is removed by immobilized neuraminidase, undergoes changes in structure and antigenicity. Such asialylated rabbit IgG's tend to agglutinate, and are immunogenic in autologous hosts. The sialic acid content of rheumatoid factor (RF) isolated from the serum of a rheumatoid patient and identified as IgG and IgM, was also found to be lower than that of normal IgG and IgM. These findings indicate that carbohydrate residues influence the secondary structure of IgG and suggest an enzymic mechanism for the genesis of RF.
源自人或兔的天然IgG，经固定化神经氨酸酶去除末端唾液酸后，其结构和抗原性会发生变化。这种去唾液酸化的兔IgG易于凝集，并且在自体宿主中具有免疫原性。从类风湿性关节炎患者血清中分离并鉴定为IgG和IgM的类风湿因子（RF）的唾液酸含量，也低于正常IgG和IgM。这些发现表明碳水化合物残基会影响IgG的二级结构，并提示了RF产生的酶促机制。
