Ascher O, Apte R N, Pluznik D H
Immunogenetics. 1981;12(1-2):117-27. doi: 10.1007/BF01561655.
Incubation of murine spleen-cell cultures with lipopolysaccharide (LPS) induces interferon (IF) production. Maximal IF levels are obtained after incubation with 100 microgram/ml for 10 h. Two inbred mouse strains differing in their ability to generate LPS-induced IF in spleen-cell cultures were used: C3H/eB, which generates high levels of IF (about 60 units/ml), and C3H/HeJ, which fails to generate detectable quantities of IF. In a genetic analysis these strains were hybridized and IF production was determined in spleen-cell cultures from F1 and F2 generations, and from backcrosses of F1 hybrids to parent strains. The results indicate that, in parent strains, a single dominant autosomal gene is responsible for differences in IF production in spleen cultures. LPS-induced IF in spleen-cell cultures resists pH 2 for as long as 48 h, but is labile to heating at 56 degrees C for 30 min. Both macrophages and lymphocytes must be present in cultures for generation of LPS-induced IF. By using mixed cultures of macrophages and lymphocytes from C3H/eB and C3H/HeJ mice, it was shown that macrophages have to interact directly with LPS to enable IF production in the cultures.
用脂多糖（LPS）培养小鼠脾细胞可诱导干扰素（IF）的产生。用100微克/毫升培养10小时后可获得最大IF水平。使用了两种在脾细胞培养中产生LPS诱导IF能力不同的近交小鼠品系：C3H/eB，能产生高水平的IF（约60单位/毫升）；C3H/HeJ，不能产生可检测量的IF。在一项遗传分析中，将这些品系进行杂交，并测定F1和F2代以及F1杂种与亲本品系回交后代的脾细胞培养物中的IF产生情况。结果表明，在亲本品系中，一个单一的显性常染色体基因负责脾培养物中IF产生的差异。脾细胞培养物中LPS诱导的IF在pH 2条件下可耐受长达48小时，但在56℃加热30分钟则不稳定。培养物中必须同时存在巨噬细胞和淋巴细胞才能产生LPS诱导的IF。通过使用来自C3H/eB和C3H/HeJ小鼠的巨噬细胞和淋巴细胞混合培养物，发现巨噬细胞必须直接与LPS相互作用才能使培养物中产生IF。
