Jones L M, Berman D T, Moreno E, Deyoe B L, Gilsdorf M J, Huber J D, Nicoletti P
J Clin Microbiol. 1980 Dec;12(6):753-60. doi: 10.1128/jcm.12.6.753-760.1980.
A radial immunodiffusion (RID) test employing a polysaccharide antigen (poly B) was compared with tests currently used in the diagnosis of bovine brucellosis. Over 1,000 sera from vaccinated and infected cattle, all of which had been examined bacteriologically, were used to determine the sensitivity and specificity of the RID, card, Rivanol, and complement fixation tests. The RID test identified 90% of the cattle that were shedding Brucella in their milk. Although the complement fixation test was more sensitive, it was less specific than the RID test in cattle vaccinated as adults with Brucella abortus strain 19. A sensitive screening test, such as the card test, in combination with the RID test could be used in diagnostic laboratories, or even in the field, with little additional expense or technical expertise. An additional advantage is that the RID could be applied to sera from adult cattle as early as 2 months after vaccination, when postvaccinal agglutinins and complement-fixing antibodies may still be present. The indirect hemolytic test was used with some of the sera and was found to be a very sensitive test which could be useful in areas of low incidence but would not be practical for large-scale testing in adult-vaccinated herds.
采用多糖抗原（聚B）的放射免疫扩散（RID）试验与目前用于诊断牛布鲁氏菌病的试验进行了比较。使用来自接种疫苗和感染牛的1000多份血清，所有血清均经过细菌学检查，以确定RID、卡片试验、利凡诺试验和补体结合试验的敏感性和特异性。RID试验鉴定出90%的牛奶中排出布鲁氏菌的牛。虽然补体结合试验更敏感，但在成年时接种流产布鲁氏菌19株疫苗的牛中，其特异性低于RID试验。一种敏感的筛查试验，如卡片试验，与RID试验结合，可用于诊断实验室，甚至在现场使用，几乎无需额外费用或专业技术知识。另一个优点是，RID可在接种疫苗后2个月尽早应用于成年牛的血清，此时疫苗接种后的凝集素和补体结合抗体可能仍然存在。对部分血清进行了间接溶血试验，发现该试验非常敏感，在发病率低的地区可能有用，但对于成年接种牛群的大规模检测不实用。
