Knight E, Fahey D
J Biol Chem. 1981 Apr 25;256(8):3609-11.
Human fibroblast interferon has been purified 2,900-fold to homogeneity. The purification is achieved in two steps by chromatography on blue Sepharose. The specific activity of the homogeneous interferon is 5 X 10(8) units/mg and the yield of biological activity has ranged from 20-40%. The interferon can exist as a monomer (Mr = 20,000) and as a dimer (Mr - 40,000). The dimer can be converted to the monomer by heating in sodium dodecyl sulfate and thioglycolic acid.
人成纤维细胞干扰素已被纯化2900倍，达到了均一性。通过在蓝色琼脂糖凝胶上进行两步层析实现了纯化。均一干扰素的比活性为5×10⁸单位/毫克，生物活性产率在20%至40%之间。干扰素可以以单体（分子量 = 20,000）和二聚体（分子量 = 40,000）的形式存在。通过在十二烷基硫酸钠和巯基乙酸中加热，二聚体可以转化为单体。
