Wilson J R, Hendrickson A E
J Comp Neurol. 1981 Apr 10;197(3):517-39. doi: 10.1002/cne.901970311.
The dorsal lateral geniculate nucleus (dLGN) of Macaca monkeys was studied by Golgi and quantitative electron microscopic (EM) methods to determine if differences in neuronal morphology exist which might correlate with the known physiological separation of X-type cells into the parvocellular and Y-type cells into the magnocellular laminae. Monocularly lid-sutured Macaca monkeys were also studied by quantitative EM methods to compare the synaptic organization within laminae innervated by the deprived and nondeprived retinae. We have divided our sample of Golgi-impregnated neurons into three groups: Types A, B, and C. Type A neurons comprise the majority of the projections cells and are quite heterogeneous in their overall morphology. Type B neurons have long dendrites with multiple appendages; some have a locally ramifying beaded axon Type C neurons are characterized by dendrites which are mainly restricted to the interlaminar zones. We found Type A and B neurons in both the parvocellular and magnocellular laminae. The cell bodies of Type C neurons lay within the interlaminar zones or the parvocellular laminae. All three types contributed dendrites to the interlaminar zones. No significant differences in Golgi morphology other than overall size were found in parvocellular or magnocellular laminae that would explain the previously demonstrated electrophysiological differences. Terminal profiles and synapses in the parvocellular, magnocellular, and interlaminar zones were classified and counted using quantitative EM methods. RSD and F terminals were most numerous in all three zones. RLP terminals were rare in the interlaminar zones. A new type of terminal, RMD was found in the magnocellular interlaminar zones. The laminar and interlaminar zones have the same overall synaptic density, but differed in types of synaptic terminals. The only quantitative difference between any of these regions was in the magnocellular laminae where the counts showed 70% more F terminals per unit area. The same quantitative methods were applied to the laminar and interlaminar zones of dLGNs from monocularly lid-sutured monkeys. We found no qualitative or quantitative difference between the synaptology of zones receiving input from the deprived retina compared to the open eye retina, nor between any regions of the dLGN in deprived monkeys compared to normal monkeys.
采用高尔基染色法和定量电子显微镜（EM）方法对猕猴的背外侧膝状核（dLGN）进行研究，以确定神经元形态是否存在差异，这些差异可能与已知的X型细胞分为小细胞层、Y型细胞分为大细胞层的生理分离有关。还采用定量EM方法对单眼眼睑缝合的猕猴进行研究，以比较由剥夺和未剥夺视网膜支配的层内的突触组织。我们将高尔基染色的神经元样本分为三组：A、B和C型。A型神经元构成投射细胞的大多数，其整体形态非常异质。B型神经元有长的树突，带有多个附属物；一些有局部分支的串珠状轴突。C型神经元的特征是树突主要局限于层间区。我们在小细胞层和大细胞层中都发现了A型和B型神经元。C型神经元的细胞体位于层间区或小细胞层内。所有三种类型都向层间区贡献树突。在小细胞层或大细胞层中，除了整体大小外，未发现高尔基形态有显著差异，这些差异可以解释先前证明的电生理差异。使用定量EM方法对小细胞层、大细胞层和层间区的终末轮廓和突触进行分类和计数。RSD和F终末在所有三个区域中最多。RLP终末在层间区很少见。在大细胞层间区发现了一种新型终末，即RMD。层状区和层间区的总体突触密度相同，但突触终末类型不同。这些区域之间唯一的定量差异在于大细胞层，该层每单位面积的F终末计数显示多70%。将相同的定量方法应用于单眼眼睑缝合猕猴的dLGN的层状区和层间区。我们发现，与未缝合眼视网膜相比，接受剥夺视网膜输入的区域的突触学在质量或数量上没有差异，与正常猕猴相比，剥夺猕猴的dLGN的任何区域之间也没有差异。
