Franzén L
Acta Pathol Microbiol Scand A. 1981 Jan;89(1):57-62. doi: 10.1111/j.1699-0463.1981.tb00187.x.
A single intraperitoneal injection of Compound 48/80 in rats causes mast cell activation which is followed by a proliferation in fibroblasts and mesothelial cells in the mesentery. The same is true of organ-cultured rat mesentery, but in cultured guinea-pig mesentery Compound 48/80 has no morphological effects on mast cells and does not induce proliferation. The relationship between mast-cell secretion and cell proliferation in surrounding cells was studied further in the investigation now presented. Proliferation was estimated (a) by cytophotometric Feulgen DNA measurements in individual fibroblast and mesothelial cell nuclei; (b) by measurements of incorporation of 3H-TdR into DNA; (c) and by estimation of mitotic frequency. Mast-cell secretion was studied morphologically and by biochemical quantitation of histamine release. Compound 48/80 is a potent mast cell activator in the rat, stimulating mast cells to secretion. The results of this study suggest that Polymyxin B, another powerful mast-cell activator, apparently chemically unrelated to Compound 48/80, also stimulates the mesenterial cells to proliferation in the rat. In the guinea-pig Compound 48/80 does not induce mast-cell secretion or proliferation in fibroblasts and mesothelial cells in the mesentery. The results give further evidence that the cell proliferation induced by Compound 48/80 and Polymyxin B in rat mesentery is causally related to mast-cell secretion.
给大鼠腹腔内单次注射化合物48/80会导致肥大细胞活化，随后肠系膜中的成纤维细胞和间皮细胞增殖。器官培养的大鼠肠系膜也是如此，但在培养的豚鼠肠系膜中，化合物48/80对肥大细胞没有形态学影响，也不会诱导增殖。在本研究中，进一步探讨了肥大细胞分泌与周围细胞增殖之间的关系。增殖的评估方法如下：(a) 通过对单个成纤维细胞和间皮细胞核进行细胞光度法Feulgen DNA测量；(b) 通过测量3H-TdR掺入DNA的量；(c) 通过估计有丝分裂频率。通过形态学和组胺释放的生化定量研究肥大细胞分泌。化合物48/80是大鼠体内一种有效的肥大细胞激活剂，能刺激肥大细胞分泌。本研究结果表明，多粘菌素B作为另一种强大的肥大细胞激活剂，显然与化合物48/80在化学结构上无关，也能刺激大鼠肠系膜细胞增殖。在豚鼠中，化合物48/80不会诱导肠系膜中肥大细胞的分泌或成纤维细胞和间皮细胞的增殖。这些结果进一步证明，化合物48/80和多粘菌素B在大鼠肠系膜中诱导的细胞增殖与肥大细胞分泌存在因果关系。
