去垢剂P40对大鼠腹膜肥大细胞体外功能的影响。
The effects of nonidet P40 on the function of rat peritoneal mast cells in vitro.
作者信息
Batchelor K W, Stanworth D R
出版信息
Br J Pharmacol. 1981 Jan;72(1):95-100. doi: 10.1111/j.1476-5381.1981.tb09110.x.
Abstract
1 Treatment of purified rat peritoneal mast cells at 37 degrees C with concentrations of the non-ionic detergent nonidet P40 (NP40) up to 0.005% (v/v) failed to reduce their viability. 2 There was a marked reduction in the histamine releasing capacity of NP40-treated mast cells upon challenge with a variety of selective (adrenocorticotrophic hormone 1-24 (Synacthen), rabbit anti-rat IgE antiserum, adenosine triphosphate (ATP) and the calcium ionophore, A 23187) and non-selective (rabbit anti-rat mast cell antiserum plus complement) histamine liberators. 3 Nonidet P40 (0.005%) was found to reduce the activity of a mast cell membrane 'ecto-enzyme', calcium-activated ATPase, by about 45% when presented at the time of its assay.
摘要
- 在37摄氏度下，用浓度高达0.005%（v/v）的非离子去污剂诺乃洗涤剂P40（NP40）处理纯化的大鼠腹膜肥大细胞，并未降低其活力。2. 用多种选择性（促肾上腺皮质激素1 - 24（合成促皮质素）、兔抗大鼠IgE抗血清、三磷酸腺苷（ATP）和钙离子载体A23187）和非选择性（兔抗大鼠肥大细胞抗血清加补体）组胺释放剂攻击NP40处理的肥大细胞时，其组胺释放能力显著降低。3. 发现诺乃洗涤剂P40（0.005%）在测定时加入，可使肥大细胞膜“外酶”钙激活ATP酶的活性降低约45%。
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