用于快速筛查空肠弯曲菌肠炎的直接免疫荧光显微镜检查。
Direct immunofluorescence microscopy for rapid screening of Campylobacter enteritis.
作者信息
Hodge D S, Prescott J F, Shewen P E
出版信息
J Clin Microbiol. 1986 Nov;24(5):863-5. doi: 10.1128/jcm.24.5.863-865.1986.
Abstract
Diagnostic use of a direct fluorescent-antibody test for detection of Campylobacter jejuni and C. coli in human fecal specimens (n = 497) was compared with detection by culturing (specificity, 99.7%; sensitivity, 40%). Conjugates were prepared from immunoglobulin G antibody against 22 Lior C. jejuni and C. coli reference strains (H. Lior, D. L. Woodward, J. A. Edgar, L. J. Laroche, and P. Gill, J. Clin. Microbiol. 15:761-768, 1982). Interestingly, the serotypes of cultures tested by the direct fluorescent antibody test were different from those of cultures tested by Lior slide agglutination, although the antisera used were common to both test systems.
摘要
将用于检测人粪便标本（n = 497）中为空肠弯曲菌和结肠弯曲菌的直接荧光抗体试验的诊断用途与通过培养进行的检测相比较（特异性为99.7%；敏感性为40%）。用针对22株利奥尔空肠弯曲菌和结肠弯曲菌参考菌株的免疫球蛋白G抗体制备结合物（H.利奥尔、D.L.伍德沃德、J.A.埃德加、L.J.拉罗什和P.吉尔，《临床微生物学杂志》15:761 - 768，1982年）。有趣的是，尽管两个检测系统使用的抗血清相同，但通过直接荧光抗体试验检测的培养物血清型与通过利奥尔玻片凝集试验检测的培养物血清型不同。
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