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用于快速筛查空肠弯曲菌肠炎的直接免疫荧光显微镜检查。

Direct immunofluorescence microscopy for rapid screening of Campylobacter enteritis.

作者信息

Hodge D S, Prescott J F, Shewen P E

出版信息

J Clin Microbiol. 1986 Nov;24(5):863-5. doi: 10.1128/jcm.24.5.863-865.1986.

DOI:10.1128/jcm.24.5.863-865.1986
PMID:3533990
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC269047/
Abstract

Diagnostic use of a direct fluorescent-antibody test for detection of Campylobacter jejuni and C. coli in human fecal specimens (n = 497) was compared with detection by culturing (specificity, 99.7%; sensitivity, 40%). Conjugates were prepared from immunoglobulin G antibody against 22 Lior C. jejuni and C. coli reference strains (H. Lior, D. L. Woodward, J. A. Edgar, L. J. Laroche, and P. Gill, J. Clin. Microbiol. 15:761-768, 1982). Interestingly, the serotypes of cultures tested by the direct fluorescent antibody test were different from those of cultures tested by Lior slide agglutination, although the antisera used were common to both test systems.

摘要

将用于检测人粪便标本(n = 497)中为空肠弯曲菌和结肠弯曲菌的直接荧光抗体试验的诊断用途与通过培养进行的检测相比较(特异性为99.7%;敏感性为40%)。用针对22株利奥尔空肠弯曲菌和结肠弯曲菌参考菌株的免疫球蛋白G抗体制备结合物(H.利奥尔、D.L.伍德沃德、J.A.埃德加、L.J.拉罗什和P.吉尔,《临床微生物学杂志》15:761 - 768,1982年)。有趣的是,尽管两个检测系统使用的抗血清相同,但通过直接荧光抗体试验检测的培养物血清型与通过利奥尔玻片凝集试验检测的培养物血清型不同。

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本文引用的文献

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NEW TRANSPORT MEDIUM FOR SHIPMENT OF CLINICAL SPECIMENS. I. FECAL SPECIMENS.用于临床标本运输的新型运输介质。一、粪便标本。
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Serotyping of Campylobacter jejuni by slide agglutination based on heat-labile antigenic factors.基于热不稳定抗原因子的玻片凝集法对空肠弯曲菌进行血清分型。
J Clin Microbiol. 1982 May;15(5):761-8. doi: 10.1128/jcm.15.5.761-768.1982.
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Comparative efficacy of liquid enrichment medium for isolation of Campylobacter jejuni.用于分离空肠弯曲菌的液体富集培养基的比较功效
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Molecular identification of surface protein antigens of Campylobacter jejuni.空肠弯曲菌表面蛋白抗原的分子鉴定
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Analysis of Campylobacter jejuni antigens with monoclonal antibodies.用单克隆抗体分析空肠弯曲菌抗原。
J Clin Microbiol. 1984 Feb;19(2):129-33. doi: 10.1128/jcm.19.2.129-133.1984.
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Correlation of an expanded direct fluorescent-antibody system with an established passive hemagglutination system for serogrouping strains of Campylobacter jejuni and Campylobacter coli.一种用于空肠弯曲菌和结肠弯曲菌菌株血清分组的扩展直接荧光抗体系统与既定被动血凝系统的相关性。
J Clin Microbiol. 1983 Nov;18(5):1064-9. doi: 10.1128/jcm.18.5.1064-1069.1983.
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Serogroups of Campylobacter jejuni, Campylobacter coli, and Campylobacter fetus defined by direct immunofluorescence.通过直接免疫荧光法确定的空肠弯曲菌、结肠弯曲菌和胎儿弯曲菌的血清群。
J Clin Microbiol. 1983 Mar;17(3):529-38. doi: 10.1128/jcm.17.3.529-538.1983.
10
Human antibody response to outer membrane proteins of Campylobacter jejuni during infection.人类感染空肠弯曲菌期间对外膜蛋白的抗体反应。
Infect Immun. 1984 Feb;43(2):739-43. doi: 10.1128/iai.43.2.739-743.1984.