Miyai K, Nagamachi E, Kobayashi Y, Watanabe F
Endocrinol Jpn. 1981 Feb;28(1):65-8. doi: 10.1507/endocrj1954.28.65.
The binding of purified 131I-3, 3', 5'-triiodothyronine (reverse T3) (rT3) to normal human serum components was investigated by a radioimmunoelectrophoretic technique. When anti-whole human serum was used, five distinct arcs of radioactivity were observed. Evidence was obtained that five of these radioactive arcs were not artifacts, but were due to components binding rT3. From the radioimmunoelectrophoretic patterns with specific antisera, five of these components were identified as thyroxine binding prealbumin, albumin, thyroxine binding globulin (TBG) and alpha 1-and beta-lipoproteins. No radioactive arc of TBG was detected in serum from a patient with TBG deficiency.
采用放射免疫电泳技术研究了纯化的131I-3,3',5'-三碘甲腺原氨酸（反三碘甲腺原氨酸，rT3）与正常人血清成分的结合情况。当使用抗全人血清时，观察到五条明显的放射性弧。有证据表明，其中五条放射性弧不是假象，而是由结合rT3的成分所致。根据使用特异性抗血清的放射免疫电泳图谱，确定其中五种成分分别为甲状腺素结合前白蛋白、白蛋白、甲状腺素结合球蛋白（TBG）以及α1-和β-脂蛋白。在一名TBG缺乏症患者的血清中未检测到TBG的放射性弧。
