Nachamkin I, Cannon J G, Mittler R S
Infect Immun. 1981 May;32(2):641-8. doi: 10.1128/iai.32.2.641-648.1981.
Hybridomas secreting monoclonal antibodies against an apparent strain-specific cell surface antigen of Neisseria gonorrhoeae were produced. Spleen cells from BALB/c mice immunized with whole gonococci were fused with mouse myeloma cell line Sp2/0, and hybrid cells were selected in culture. One hybridoma that secreted antibodies reactive with the immunizing strain was cloned by limiting dilution to obtain cell lines secreting monoclonal antibodies. These antibodies reacted with purified outer membranes from the immunizing strain as well as with whole gonococci. Binding of antibodies to whole gonococci was highly strain specific, with most gonococcal strains showing less than 1% of the binding with the immunizing strain. Antibodies did not bind to the other Neisseria species tested. Binding of monoclonal antibodies to whole gonococci of the immunizing strain was not dependent on state of piliation. The extent of antibody binding did vary in different colonial variants of the immunizing strain. Antibody bound to cells from colonies that were transparent or of intermediate opacity, but did not bind to cells from deeply opaque colony variants.
制备了分泌针对淋病奈瑟菌一种明显的菌株特异性细胞表面抗原的单克隆抗体的杂交瘤。用全菌免疫的BALB/c小鼠的脾细胞与小鼠骨髓瘤细胞系Sp2/0融合，并在培养中筛选杂交细胞。通过有限稀释法克隆出一株分泌与免疫菌株反应性抗体的杂交瘤，以获得分泌单克隆抗体的细胞系。这些抗体与免疫菌株的纯化外膜以及全菌发生反应。抗体与全菌的结合具有高度菌株特异性，大多数淋球菌菌株与免疫菌株的结合率不到1%。抗体不与所测试的其他奈瑟菌属物种结合。单克隆抗体与免疫菌株全菌的结合不依赖于菌毛状态。在免疫菌株的不同菌落变体中，抗体结合程度有所不同。抗体与透明或中等不透明度菌落的细胞结合，但不与深不透明菌落变体的细胞结合。
