Apicella M A
J Infect Dis. 1976 Oct;134(4):377-83. doi: 10.1093/infdis/134.4.377.
A series of population-specific acidic polysaccharides have been described that can be used as a basis for serogrouping of Neisseria gonorrhoeae. These polysaccharides have been designated Gc antigens, and four immunologically distinct types have been identified. With these purified serogroup antigens and appropriately absorbed antisera in hemagglutination-inhibition systems, four typing systems have been established. Their sensitivities for purified homologous antigens range from 16 to 1 mug/ml. Purified heterologus antigens fail to inhibit at concentrations of 1,000 mug/ml. Clinically isolated N. gonorrhoeae are incorporated into these systems by conversion to standardized crude Gc antigen extracts by alkaline hhydrolysis. Of the 163 strains studied, 83% could be typed; 85% of these were typed for only one serogroup. Twenty strains were typed for two serogroups, and reisolation studies demonstrated that these strains were mosaics rather than mixed cultures. Four strains from each serogroup were selected, and antisera and purified serogroup antigens were produced from them. These were identical with their respective standard serogroup antigen and antisera in hemagglutination-inhibition and immunodiffusion systems.
已描述了一系列特定人群的酸性多糖，它们可作为淋病奈瑟菌血清分组的基础。这些多糖被指定为Gc抗原，已鉴定出四种免疫上不同的类型。利用这些纯化的血清群抗原和在血凝抑制系统中适当吸收的抗血清，建立了四种分型系统。它们对纯化的同源抗原的敏感性范围为16至1微克/毫升。纯化的异源抗原在1000微克/毫升的浓度下不能产生抑制作用。临床分离的淋病奈瑟菌通过碱性水解转化为标准化的粗Gc抗原提取物后被纳入这些系统。在研究的163株菌株中，83%可以分型；其中85%仅被分型为一个血清群。20株菌株被分型为两个血清群，重新分离研究表明这些菌株是嵌合体而非混合培养物。从每个血清群中选择四株菌株，并从它们中制备抗血清和纯化的血清群抗原。这些在血凝抑制和免疫扩散系统中与各自的标准血清群抗原和抗血清相同。
