Comparison of manually performed Microtiter plate and semiautomated (cuvette) indirect enzyme-linked immunosorbent assays for the serodiagnosis of mycoplasmal pneumonia of swine.

Standardized enzyme-linked immunosorbent assays for the serodiagnosis of mycoplasmal pneumonia of swine were developed, using the traditional Microtiter plate method and a method based on semiautomated processor-analyzer instrumentation. The results of the 2 procedures were not significantly different when the titers of positive and negative anti-Mycoplasma hyopneumoniae standard reference sera were determined in triplicate in 3 separate experiments. Although both methods yielded reproducible results, the instrument-assisted enzyme-linked immunosorbent assay would be preferable if large numbers of samples are to be tested.