Retinal neovascularization after intravitreal fibroblast injection.

We have developed a new model of retinal neovascularization in the rabbit. After intravitreal autotransplantation of 250,000 tissue-cultured skin fibroblasts, we observed strand formation in the midvitreous cavity. These strands grew toward the vascularized part of the retina and attached to it. Once the attachment was established, retinal neovascularization began with growth of vessels along the fibrous strand. After three months, this retinal neovascularization regressed. The presence of immature capillaries was confirmed by electron microscopic examination. Of 43 eyes, 31 (72%) developed neovascularization.