Mulligan P K
J Exp Zool. 1981 Jul;217(1):109-18. doi: 10.1002/jez.1402170112.
The female sterile mutant of Drosophila melanogaster, fs(1)1304 (1-19 +/- 2), has been characterized. Our studies show that the mutation affects the organization of nucleolar material in the ovarian nurse cells and the pattern of RNA metabolism in the ovary. Autoradiographic analysis of incorporation of 3H-uridine in vivo and analysis of 3H-uridine incorporation into high molecular weight RNA in vitro suggest that RNA from the ovaries of homozygous fs flies is degraded at a higher rate than that from heterozygous fs and wild-type ovaries. It is likely that the RNA class affected is ribosomal RNA. These data are discussed in the context of the functional role for the wild-type gene allelic to fs(1)1304, and it is suggested that one of the effects of the mutation may be on the biogenesis of ribosomes that are to be stored in the oocyte.
已对黑腹果蝇的雌性不育突变体fs(1)1304(1-19±2)进行了表征。我们的研究表明，该突变影响卵巢滋养细胞中核仁物质的组织以及卵巢中RNA代谢模式。体内3H-尿苷掺入的放射自显影分析和体外3H-尿苷掺入高分子量RNA的分析表明，纯合fs果蝇卵巢中的RNA降解速度高于杂合fs和野生型卵巢中的RNA。受影响的RNA类别可能是核糖体RNA。这些数据在与fs(1)1304等位的野生型基因的功能作用的背景下进行了讨论，并且表明该突变的影响之一可能在于将存储在卵母细胞中的核糖体的生物发生。
