Giorno R, Beverly S
Stain Technol. 1981 May;56(3):189-93. doi: 10.3109/10520298109067309.
A method for rapidly determining levels of T lymphocytes in humans is presented. The method involves staining lymphocytes in peripheral blood or buffy coat smears for acid alpha-naphthylacetate esterase for 1 hr at 37 C. After counterstaining with Wright's stain, the percentage of esterase-positive lymphocytes accurately reflects the percentage of lymphocytes forming E rosettes with sheep erythrocytes.
本文介绍了一种快速测定人体T淋巴细胞水平的方法。该方法包括在37℃下将外周血或血沉棕黄层涂片上的淋巴细胞用酸性α-萘乙酸酯酶染色1小时。用瑞氏染色复染后,酯酶阳性淋巴细胞的百分比准确反映了与绵羊红细胞形成E花环的淋巴细胞的百分比。