用酸性α-萘乙酸酯酶测定T淋巴细胞水平的快速方法。
Rapid method for determining T lymphocyte levels using acid alpha-naphthyl acetate esterase.
作者信息
Giorno R, Beverly S
出版信息
Stain Technol. 1981 May;56(3):189-93. doi: 10.3109/10520298109067309.
PMID:6168040
Abstract
A method for rapidly determining levels of T lymphocytes in humans is presented. The method involves staining lymphocytes in peripheral blood or buffy coat smears for acid alpha-naphthylacetate esterase for 1 hr at 37 C. After counterstaining with Wright's stain, the percentage of esterase-positive lymphocytes accurately reflects the percentage of lymphocytes forming E rosettes with sheep erythrocytes.
摘要
本文介绍了一种快速测定人体T淋巴细胞水平的方法。该方法包括在37℃下将外周血或血沉棕黄层涂片上的淋巴细胞用酸性α-萘乙酸酯酶染色1小时。用瑞氏染色复染后，酯酶阳性淋巴细胞的百分比准确反映了与绵羊红细胞形成E花环的淋巴细胞的百分比。
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