Wright G L, Schellhammer P F, Brassil D N, Sieg S M, Leffell M S
Clin Chem. 1981 Oct;27(10):1747-52.
We evaluated and compared five commercial radioimmunoassay kits with a standard counter-immunoelectrophoretic assay for the measurement of prostatic acid phosphatase in serum. Four of the five radioimmunoassays performed as described by the supplier with respect to sensitivity, stability, precision, linearity, analytical recovery, and expected values for the normal male population. None of the radioimmunoassays was more clinically sensitive then the counter-immunoelectrophoretic assay for detecting increased prostatic acid phosphatase in serum. Results obtained by counter-immunoelectrophoretic assay agreed with results obtained by radioimmunoassay in 96% of the tests. The proportion of positive results in patients with confirmed prostatic adenocarcinoma increased with disease progression. The fewer positive tests in localized adenocarcinoma (Stages A and B) suggests that neither the counter-immunoelectrophoretic assay nor the radioimmunoassay procedures are useful for screening unselected populations for adenocarcinoma of the prostate. The high percentage of normal values found in those patients clinically free of disease after treatment is encouraging and supports the use of the prostatic acid phosphatase immunoassays in prospectively monitoring the treatment of prostatic cancer patients.
我们用标准的对流免疫电泳法对五种市售放射免疫分析试剂盒进行了评估和比较，以测定血清中的前列腺酸性磷酸酶。五种放射免疫分析中有四种在灵敏度、稳定性、精密度、线性、分析回收率以及正常男性群体的预期值方面的表现与供应商描述的一致。在检测血清中前列腺酸性磷酸酶升高方面，没有一种放射免疫分析比对流免疫电泳法在临床上更敏感。对流免疫电泳法得到的结果与放射免疫分析得到的结果在96%的检测中一致。确诊为前列腺腺癌的患者中阳性结果的比例随疾病进展而增加。局限性腺癌（A期和B期）中阳性检测较少，这表明对流免疫电泳法和放射免疫分析程序都不适用于对未选择的人群进行前列腺腺癌筛查。在治疗后临床上无疾病的患者中发现高比例的正常值令人鼓舞，并支持使用前列腺酸性磷酸酶免疫分析法对前列腺癌患者进行前瞻性治疗监测。
