对流免疫电泳法测定人血清中前列腺酸性磷酸酶
Counterimmunoelectrophoresis in determination of prostatic acid phosphatase in human serum.
作者信息
Foti A G, Cooper J F, Herschman H
出版信息
Clin Chem. 1978 Jan;24(1):140-2.
We evaluated counterimmunoelectrophoresis for use in measuring prostatic acid phosphatase in detection of prostatic cancer. After staining for acid phosphatase, we could detect as little as 0.3 ng of purified enzyme standard complexed with antibody by this technique. However, when serum samples were used as antigen, the method was less sensitive (1.5-2.0 ng) because some of the serum proteins migrate with the phosphatase and decrease the intensity of the stain for acid phosphatase. For this reason we could not detect the phosphatase in serum samples of normal persons; only patients with moderately (or greater) increased activity in their serum showed positive results. In contrast, by radioimmunoassay as little as 1.0 ng of the phosphatase can be detected in serum.
我们评估了对流免疫电泳法在检测前列腺癌时用于测量前列腺酸性磷酸酶的情况。在用酸性磷酸酶染色后，通过该技术我们能够检测到低至0.3纳克与抗体复合的纯化酶标准品。然而，当使用血清样本作为抗原时，该方法的灵敏度较低（1.5 - 2.0纳克），因为一些血清蛋白会与磷酸酶一起迁移，降低酸性磷酸酶染色的强度。因此，我们无法在正常人的血清样本中检测到磷酸酶；只有血清中活性中度（或更高）升高的患者显示出阳性结果。相比之下，通过放射免疫测定法，血清中低至1.0纳克的磷酸酶就能被检测到。
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