Gupta M K, Rajaraman S, Gupta S K, Hewitt C B, Deodhar S D
Am J Clin Pathol. 1981 Oct;76(4):430-6. doi: 10.1093/ajcp/76.4.430.
Two radioimmunoassay procedures (RIA-1 and RIA-2) were evaluated for the quantitation of prostatic acid phosphatase in serum and compared with the enzymatic method and counter immunoelectrophoresis method for their specificity and sensitivity. Sera from 168 patients were analyzed and these included: normals, 27; untreated prostatic cancer patients Stage A, 2; Stage C, 3; Stage D, 17; cancer of prostate treated with different modalities, 42; sarcoma of prostate, 1; prostatitis, 3; nonprostatic carcinoma, 17; and benign prostatic hyperplasia (BPH), 56. RIA-1 procedure appeared more sensitive (82% sensitivity) and specific (94.5% specificity) than the RIA-2 procedure (68% sensitivity and 91.8% specificity), but the differences were not statistically significant. The enzymatic method was found to be least sensitive (63.6% sensitivity) but also the most specific (100% specificity). Only 69 of the specimens were analyzed by counter immunoelectrophoresis, which showed sensitivity of 87% and specificity of 51.4%. False positives were observed more often in patients with nonprostatic cancer and BPH. The variations in diagnostic specificity of immunologic assays suggest the need of characterization of each antibody specificity.
评估了两种放射免疫分析方法（RIA-1和RIA-2）用于血清中前列腺酸性磷酸酶的定量分析，并将其与酶法和对流免疫电泳法在特异性和敏感性方面进行了比较。分析了168例患者的血清，这些患者包括：正常人27例；未经治疗的前列腺癌患者，A期2例、C期3例、D期17例；接受不同治疗方式的前列腺癌患者42例；前列腺肉瘤1例；前列腺炎3例；非前列腺癌17例；良性前列腺增生（BPH）56例。RIA-1方法似乎比RIA-2方法更敏感（敏感性82%）和更具特异性（特异性94.5%），而RIA-2方法的敏感性为68%，特异性为91.8%，但差异无统计学意义。发现酶法最不敏感（敏感性63.6%），但特异性最高（特异性100%）。仅69份标本采用对流免疫电泳进行分析，其敏感性为87%，特异性为51.4%。非前列腺癌患者和BPH患者中假阳性更为常见。免疫分析诊断特异性的差异表明需要对每种抗体的特异性进行表征。
