Macnaughton M R, Madge M H, Reed S E
Infect Immun. 1981 Sep;33(3):734-7. doi: 10.1128/iai.33.3.734-737.1981.
Paired sera from volunteers inoculated with one of the five recently isolated strains of human coronavirus (HCV), AD, GI, HO, PA, and RO, none of which has been grown in tissue culture, or with strain OC38 were tested against coronavirus antigens by enzyme-linked immunosorbent assay. When HCV strains OC43, 229E, or the 229E-related tissue culture-adapted strains PR and TO were used as antigens, it was shown that all strains fell into one of two antigenic groups. The HCV OC43 group was comprised of strains OC43, GI, HO, and RO, and the HCV 229E group contained strains AD and PA as well as the tissue culture-adapted strains PR, TO, and KI. Enzyme-linked immunosorbent assay of the paired sera with the coronavirus mouse hepatitis virus strain 3 as antigen confirmed the relationship of this virus to the HCV OC43 group but not to the HCV 229E group.
对接种了最近分离出的五种人类冠状病毒（HCV）毒株之一（AD、GI、HO、PA和RO，均未在组织培养中培养）或OC38毒株的志愿者的配对血清，通过酶联免疫吸附测定法检测其针对冠状病毒抗原的情况。当使用HCV毒株OC43、229E或与229E相关的适应组织培养的毒株PR和TO作为抗原时，结果表明所有毒株可分为两个抗原组之一。HCV OC43组由毒株OC43、GI、HO和RO组成，HCV 229E组包含毒株AD和PA以及适应组织培养的毒株PR、TO和KI。以冠状病毒小鼠肝炎病毒毒株3作为抗原对配对血清进行酶联免疫吸附测定，证实了该病毒与HCV OC43组的关系，但与HCV 229E组无关。
