使用酶联免疫吸附测定法诊断儿童人类冠状病毒感染
Diagnosis of human coronavirus infections in children using enzyme-linked immunosorbent assay.
作者信息
Macnaughton M R, Flowers D, Isaacs D
出版信息
J Med Virol. 1983;11(4):319-25. doi: 10.1002/jmv.1890110407.
Abstract
An enzyme-linked immunosorbent assay (ELISA) was developed for diagnosing human coronavirus (HCV) infections in children. One hundred and seventy seven nose swabs, throat swabs, and nasopharyngeal aspirates were collected from 30 children suffering from acute respiratory infections. These samples were tested for HCV antigens by ELISA and 28.2% of the samples were shown to be HCV positive. These results indicate that our ELISA should prove useful in the diagnosis of HCV infections in children. Further studies are in progress to extend the ELISA to detect HCVs in experimentally and naturally acquired infections in adults.
摘要
已开发出一种酶联免疫吸附测定法（ELISA）用于诊断儿童人类冠状病毒（HCV）感染。从30名患有急性呼吸道感染的儿童中收集了177份鼻拭子、咽拭子和鼻咽抽吸物。通过ELISA对这些样本进行HCV抗原检测，结果显示28.2%的样本为HCV阳性。这些结果表明，我们的ELISA在诊断儿童HCV感染方面应会证明是有用的。正在进行进一步研究，以扩展该ELISA用于检测成人实验性和自然获得性感染中的HCV。
相似文献
1
Diagnosis of human coronavirus infections in children using enzyme-linked immunosorbent assay.使用酶联免疫吸附测定法诊断儿童人类冠状病毒感染
J Med Virol. 1983;11(4):319-25. doi: 10.1002/jmv.1890110407.
2
Diagnosis of human coronavirus infection by immunofluorescence: method and application to respiratory disease in hospitalized children.通过免疫荧光法诊断人冠状病毒感染：方法及其在住院儿童呼吸道疾病中的应用
J Med Virol. 1978;2(4):341-6. doi: 10.1002/jmv.1890020407.
3
4
Enzyme-linked immunosorbent assay for detection of respiratory syncytial virus infection: application to clinical samples.用于检测呼吸道合胞病毒感染的酶联免疫吸附测定：在临床样本中的应用
J Clin Microbiol. 1982 Aug;16(2):329-33. doi: 10.1128/jcm.16.2.329-333.1982.
5
Enzyme-linked immunosorbent assay for the detection of canine coronavirus and its antibody in dogs.用于检测犬冠状病毒及其抗体的酶联免疫吸附测定法。
Vet Microbiol. 1991 Jan;26(1-2):41-51. doi: 10.1016/0378-1135(91)90040-m.
6
Isolation of bovine coronavirus from feces and nasal swabs of calves with diarrhea.从腹泻犊牛的粪便和鼻拭子中分离牛冠状病毒。
J Vet Med Sci. 1991 Jun;53(3):433-7. doi: 10.1292/jvms.53.433.
7
Use of nasal and pharyngeal swabs for rapid detection of respiratory syncytial virus and adenovirus antigens by enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法使用鼻拭子和咽拭子快速检测呼吸道合胞病毒和腺病毒抗原
J Clin Microbiol. 1989 Aug;27(8):1867-9. doi: 10.1128/jcm.27.8.1867-1869.1989.
8
[Evaluation of serum specific IgM detection in diagnosis of respiratory viral infections in children].[血清特异性IgM检测在儿童呼吸道病毒感染诊断中的评估]
Zhonghua Er Ke Za Zhi. 2012 Jun;50(6):440-4.
9
Evaluation of ELISA and electron microscopy for the detection of coronavirus and rotavirus in bovine faeces.酶联免疫吸附测定法（ELISA）和电子显微镜技术用于检测牛粪便中冠状病毒和轮状病毒的评估
Vet Rec. 1984 Apr 21;114(16):397-401. doi: 10.1136/vr.114.16.397.
10
Enzyme-linked immunosorbent assay for detection of antibody in volunteers experimentally infected with human coronavirus strain 229 E.用于检测实验感染人冠状病毒229E株志愿者体内抗体的酶联免疫吸附测定法。
J Clin Microbiol. 1980 Oct;12(4):493-7. doi: 10.1128/jcm.12.4.493-497.1980.
引用本文的文献
1
Viral pneumonia in children.儿童病毒性肺炎
Semin Pediatr Infect Dis. 1998 Jul;9(3):217-233. doi: 10.1016/S1045-1870(98)80035-6. Epub 2006 Jun 3.
2
The clinical presentation and outcomes of children infected with newly identified respiratory tract viruses.新发现的呼吸道病毒感染儿童的临床表现及预后
Infect Dis Clin North Am. 2005 Sep;19(3):569-84. doi: 10.1016/j.idc.2005.05.009.
3
Antigen detection in human respiratory Coronavirus infections by monoclonal time-resolved fluoroimmunoassay.采用单克隆时间分辨荧光免疫分析法检测人呼吸道冠状病毒感染中的抗原
Clin Diagn Virol. 1994 Jun;2(3):165-79. doi: 10.1016/0928-0197(94)90020-5.
4
[Nosocomial infections due to human coronaviruses in the newborn].[新生儿中由人类冠状病毒引起的医院感染]
Arch Pediatr. 2002 Jan;9(1):61-9. doi: 10.1016/s0929-693x(01)00696-0.
5
Comparison of immunofluorescence with monoclonal antibodies and RT-PCR for the detection of human coronaviruses 229E and OC43 in cell culture.在细胞培养中使用单克隆抗体免疫荧光法与逆转录聚合酶链反应检测人冠状病毒229E和OC43的比较
J Virol Methods. 1998 Jun;72(2):145-52. doi: 10.1016/s0166-0934(98)00013-5.
6
Development and application of an enzyme immunoassay for coronavirus OC43 antibody in acute respiratory illness.急性呼吸道疾病中冠状病毒OC43抗体酶免疫测定法的开发与应用。
J Clin Microbiol. 1994 Oct;32(10):2372-6. doi: 10.1128/jcm.32.10.2372-2376.1994.
7
Antigenic characterization of human coronaviruses 229E and OC43 by enzyme-linked immunosorbent assay.通过酶联免疫吸附测定对人冠状病毒229E和OC43进行抗原特性分析。
J Clin Microbiol. 1984 Aug;20(2):175-80. doi: 10.1128/jcm.20.2.175-180.1984.
8
Enzyme-linked immunoassays for the detection of microbial antigens and their antibodies.用于检测微生物抗原及其抗体的酶联免疫测定法。
Adv Appl Microbiol. 1986;31:271-92. doi: 10.1016/s0065-2164(08)70445-3.
9
Rapid and accurate viral diagnosis.快速准确的病毒诊断。
Pharmacol Ther. 1989;40(2):287-328. doi: 10.1016/0163-7258(89)90098-3.
10
Characterization of a nucleic acid probe for the diagnosis of human coronavirus 229E infections.用于诊断人冠状病毒229E感染的核酸探针的特性分析。
J Med Virol. 1990 Jun;31(2):165-72. doi: 10.1002/jmv.1890310216.
本文引用的文献
1
CULTIVATION OF A NOVEL TYPE OF COMMON-COLD VIRUS IN ORGAN CULTURES.新型普通感冒病毒在器官培养中的培养
Br Med J. 1965 Jun 5;1(5448):1467-70. doi: 10.1136/bmj.1.5448.1467.
2
Structural and antigenic relationships between human, murine and avian coronaviruses.人、鼠和禽冠状病毒之间的结构与抗原关系。
Adv Exp Med Biol. 1981;142:19-28. doi: 10.1007/978-1-4757-0456-3_2.
3
Prevalence of human coronavirus antibody in the population of southern Iraq.伊拉克南部人群中人类冠状病毒抗体的流行情况。
J Med Virol. 1982;9(3):209-16. doi: 10.1002/jmv.1890090308.
4
Infectivity of human coronavirus strain 229E.人冠状病毒229E株的传染性。
J Clin Microbiol. 1980 Sep;12(3):462-8. doi: 10.1128/jcm.12.3.462-468.1980.
5
Occurrence and frequency of coronavirus infections in humans as determined by enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法确定的人类冠状病毒感染的发生率和频率。
Infect Immun. 1982 Nov;38(2):419-23. doi: 10.1128/iai.38.2.419-423.1982.
6
Selective infection of lower respiratory tract by respiratory viruses in children with recurrent respiratory tract infections.反复呼吸道感染患儿呼吸道病毒对下呼吸道的选择性感染
Br Med J (Clin Res Ed). 1982 Jun 12;284(6331):1746-8. doi: 10.1136/bmj.284.6331.1746.
7
Isolation of rhinoviruses and coronaviruses from 38 colds in adults.从38例成人感冒病例中分离出鼻病毒和冠状病毒。
J Med Virol. 1980;5(3):221-9. doi: 10.1002/jmv.1890050306.
8
[An epidemic of ulceronecrotizing enterocolitis in a maternity ward. Arguments in favor of its viral origin].[产科病房的坏死性溃疡性小肠结肠炎疫情。支持其病毒起源的论据]
Bull Acad Natl Med. 1980 Mar;164(3):286-93.
9
Enzyme-linked immunosorbent assay for coronaviruses HCV 229E and MHV 3.针对冠状病毒HCV 229E和MHV 3的酶联免疫吸附测定。
J Gen Virol. 1980 Jul;49(1):83-9. doi: 10.1099/0022-1317-49-1-83.
10
Enzyme-linked immunosorbent assay for detection of antibody in volunteers experimentally infected with human coronavirus strain 229 E.用于检测实验感染人冠状病毒229E株志愿者体内抗体的酶联免疫吸附测定法。
J Clin Microbiol. 1980 Oct;12(4):493-7. doi: 10.1128/jcm.12.4.493-497.1980.
Zotero 插件