Sehgal P B, Sagar A D, Braude I A
Science. 1981 Nov 13;214(4522):803-5. doi: 10.1126/science.6170112.
Translationally active (in Xenopus oocytes) human alpha interferon (IFN) messenger RNA's (mRNA's) derived from Sendai virus--induced leukocyte cultures display a bimodal distribution of RNA lengths on electrophoresis through agarose-CH3HgOH gels. The major population (alpha s) consists of mRNA of length 0.7 to 1.4 kilobases, while the minor population (alpha L) consists of RNA of length 1.6 to 3.5 kilobases. Induction of human leukocytes in the presence of 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB; 100 micromolar) appears to inhibit the accumulation of IFN-alpha s and to enhance that of IFN-alpha L mRNA's (average length about 1.8 kilobases in preparations from DRB-treated cells). Interferons derived from the alpha s mRNA's represent the group of previously recognized alpha interferons while the alpha L interferons are distinguishable from this group by their lower heterospecific activity on bovine cells compared to human cells, their apparent slower mobility in sodium dodecyl sulfate--polyacrylamide gels, and their apparent heteroclitic response toward an antiserum to IFN-alpha.
来源于仙台病毒诱导的白细胞培养物的具有翻译活性（在非洲爪蟾卵母细胞中）的人α干扰素（IFN）信使核糖核酸（mRNA），在通过琼脂糖-甲基汞凝胶进行电泳时，呈现出RNA长度的双峰分布。主要群体（αs）由长度为0.7至1.4千碱基的mRNA组成，而次要群体（αL）由长度为1.6至3.5千碱基的RNA组成。在5,6-二氯-1-β-D-呋喃核糖基苯并咪唑（DRB；100微摩尔）存在的情况下诱导人白细胞，似乎会抑制IFN-αs的积累，并增强IFN-αL mRNA的积累（来自DRB处理细胞的制剂中平均长度约为1.8千碱基）。来源于αs mRNA的干扰素代表先前公认的α干扰素群体，而αL干扰素与该群体的区别在于，与人类细胞相比，它们对牛细胞的异种特异性活性较低，在十二烷基硫酸钠-聚丙烯酰胺凝胶中迁移明显较慢，以及它们对IFN-α抗血清的明显异质性反应。
