Tripathi R K, Misra R S
Appl Environ Microbiol. 1981 Sep;42(3):389-93. doi: 10.1128/aem.42.3.389-393.1981.
The treatment of germinating maize seeds (cv. Ganga 2) with aflatoxin B1 resulted in suppression of ribonucleic acid (RNA), protein, and deoxyribonucleic acid (DNA) synthesis at 3, 4, and 5 h, respectively. At or below the concentrations inhibitory for these in vivo syntheses, the toxin inhibited chromatin-bound DNA-dependent RNA polymerase activity. The synthesis of both polyadenylated and non-polyadenylated RNA was inhibited, but the effect on the former was more pronounced. Equilibrium dialysis and difference spectral and viscometric analyses showed a binding of aflatoxin B1 to DNA isolated from the seeds. It is proposed that the inhibition of RNA synthesis in maize seeds by the toxin is due to the interference with the RNA polymerase activity, which seems, at least partially, due to the impairment of DNA template functions.
用黄曲霉毒素B1处理萌发的玉米种子（品种Ganga 2），分别在3小时、4小时和5小时导致核糖核酸（RNA）、蛋白质和脱氧核糖核酸（DNA）合成受到抑制。在抑制这些体内合成的浓度及以下，该毒素抑制了与染色质结合的依赖DNA的RNA聚合酶活性。聚腺苷酸化RNA和非聚腺苷酸化RNA的合成均受到抑制，但对前者的影响更为明显。平衡透析、差示光谱分析和粘度分析表明，黄曲霉毒素B1与从种子中分离的DNA结合。有人提出，该毒素对玉米种子RNA合成的抑制是由于对RNA聚合酶活性的干扰，这似乎至少部分是由于DNA模板功能的损害。
