Garbutt G J, Abraham E C
Biochim Biophys Acta. 1981 Sep 29;670(2):190-4. doi: 10.1016/0005-2795(81)90008-8.
Chromatographically purified Hb F0, Hb A0 and Hb S0 fractions were incubated with 70 microM [14C]acetyl-CoA for 3 h in 20 mM Tris-HCl, pH 7.6 and 8.6. The acid-precipitable radioactivity was monitored and the hemoglobins were separated by Biorex 70 chromatography. The pH dependence of acetylation showed an increase in acetylation with an increase in pH from 7.6 to 8.6, whereas an increase in ionic strength decreased acetylation. Incubation of Hb F0 with [14C]acetyl-CoA resulted in modified hemoglobin and gamma chains that co-chromatographed with Hb FIc and gamma 1c chains, respectively. Acetylation of Hb A0 and Hb S0 produced minor hemoglobins whose chromatographic mobilities were slightly faster than those of Hb AIc and Hb SIc, respectively, Radioactivity peaks also appeared at the leading edges of the major hemoglobin zones as well, which indicates that, like non-enzymatic glycosylation, non-enzymatic acetylation of hemoglobins involves both specific and nonspecific reactions.
将经色谱法纯化的Hb F0、Hb A0和Hb S0组分在20 mM Tris-HCl（pH 7.6和8.6）中与70微摩尔[14C]乙酰辅酶A孵育3小时。监测酸沉淀放射性，并通过Biorex 70色谱法分离血红蛋白。乙酰化的pH依赖性表明，随着pH从7.6增加到8.6，乙酰化增加，而离子强度增加则降低乙酰化。Hb F0与[14C]乙酰辅酶A孵育产生了分别与Hb FIc和γ1c链共色谱的修饰血红蛋白和γ链。Hb A0和Hb S0的乙酰化产生了次要血红蛋白，其色谱迁移率分别略快于Hb AIc和Hb SIc。放射性峰也出现在主要血红蛋白区的前沿，这表明，与非酶糖基化一样，血红蛋白的非酶乙酰化涉及特异性和非特异性反应。
