Abraham E C
Biochim Biophys Acta. 1981 Jan 30;667(1):168-76. doi: 10.1016/0005-2795(81)90077-5.
Human adult red cell lysate contains glycosylated minor hemoglobins AIa1, AIa2, AIb, and AIc. Similar minor hemoglobins, designated FIa1, FIa2, Fib, and FIc, have been separated by a Biorex 70 column chromatographic procedure from red cell lysates of newborn children and from an adult homozygote for hereditary persistence of fetal Hb. The minor Hb components were characterized by analyzing for carbohydrate and phosphate contents, by oxygen equilibrium analysis, and by comparing the chromatographic elution profiles of naturally occurring and in vitro synthesized minor components. The results indicate that Hb FIa1, Hb FIa2, and Hb FIc have been formed by the modification of gamma chains of Hb F by reacting with fructose-1,6-P2, glucose-6-P, and glucose, respectively. Hb FIb is a glycoprotein; the mechanism of its formation is unclear. Hb FIa1 and Hb FIa2 had significantly lower oxygen affinities and n values than the other minor components and the major Hb F0. Moreover, 2,3-diphosphoglycerate did not influence the oxygenation of the minor or the major fetal Hb components. Incubations of Hb F with [14C]hexoses and subsequent chromatographic separation of hemoglobins and their globin chains confirm the previous findings that the binding of carbohydrate to Hb involves both specific and nonspecific reactions.
成人红细胞裂解液含有糖基化的次要血红蛋白AIa1、AIa2、AIb和AIc。通过Bio-Rex 70柱色谱法，已从新生儿的红细胞裂解液以及一名胎儿血红蛋白遗传性持续存在的成人纯合子中分离出了类似的次要血红蛋白，分别命名为FIa1、FIa2、Fib和FIc。通过分析碳水化合物和磷酸盐含量、进行氧平衡分析以及比较天然存在的和体外合成的次要成分的色谱洗脱图谱，对这些次要血红蛋白成分进行了表征。结果表明，Hb FIa1、Hb FIa2和Hb FIc分别是由Hb F的γ链与果糖-1,6-P2、葡萄糖-6-P和葡萄糖反应修饰而成。Hb FIb是一种糖蛋白，其形成机制尚不清楚。与其他次要成分和主要的Hb F0相比，Hb FIa1和Hb FIa2的氧亲和力和n值显著较低。此外，2,3-二磷酸甘油酸不影响次要或主要胎儿血红蛋白成分的氧合作用。用[14C]己糖孵育Hb F，随后对血红蛋白及其珠蛋白链进行色谱分离，证实了先前的发现，即碳水化合物与Hb的结合涉及特异性和非特异性反应。
