Borg H, Lundgren E, Hollenberg M D, Veale W L, Tan Y H, McPherson A
Can J Physiol Pharmacol. 1981 Aug;59(8):890-2. doi: 10.1139/y81-134.
Commonly used preparations of human interferon are pyrogenic when used in humans for clinical trials. We describe a simple two-step procedure for the purification of human fibroblast interferon, employing Blue Sepharose chromatography and high-speed centrifugation. Such preparations are devoid of pyrogenic activity in a rabbit test system and are much more suitable for use in human clinical trials than previously used preparations.
常用的人干扰素制剂在用于人体临床试验时会引起发热。我们描述了一种简单的两步法来纯化人成纤维细胞干扰素，采用蓝色琼脂糖凝胶层析和高速离心。这种制剂在兔试验系统中无发热活性，比以前使用的制剂更适合用于人体临床试验。
