Alm E, Bloom G D, Fredriksson B A
Agents Actions. 1984 Apr;14(3-4):370-2. doi: 10.1007/BF01973830.
The Seragen Cell-ect Total Histamine KitTM was used to separate histamine receptor-bearing rat peritoneal mast cells from cells lacking these receptors. It was found that approximately 75% of the peritoneal mast cell population carried cell surface histamine receptors. The results further suggest that the mast cell histamine receptors present are mainly of an H1-type, as judged by the capacity of a specific H1-antagonist to reduce the histamine receptor-dependent cell adhesion. Moreover, an H1-agonist is less efficient in this respect and an H2-antagonist does not affect the cell adhesion at all. A possible functional role for these receptors, however, remains to be clarified.
使用Seragen细胞外总组胺试剂盒从缺乏这些受体的细胞中分离出带有组胺受体的大鼠腹膜肥大细胞。发现大约75%的腹膜肥大细胞群体携带细胞表面组胺受体。结果进一步表明，根据特定H1拮抗剂减弱组胺受体依赖性细胞黏附的能力判断，目前存在的肥大细胞组胺受体主要是H1型。此外，H1激动剂在这方面效率较低，而H2拮抗剂根本不影响细胞黏附。然而，这些受体的可能功能作用仍有待阐明。
