Paterson P Y, Day E D, Whitacre C C, Berenberg R A, Harter D H
J Neurol Sci. 1981 Oct;52(1):37-51. doi: 10.1016/0022-510x(81)90132-5.
Sera of normal subjects and patients wtih multiple sclerosis (MS) have been frequently found to contain picomolar quantities of endogenous myelin basic protein-serum factors (MBP-SFs). These serum factors, collectively representing a heterogeneous spectrum, were detected and measured by means of a competitive inhibition radioimmunoassay (RIA) designed to distinguish their respective binding affinities with anti-MBP reagent antiserum. Anti-MBP antibodies in these same normal and patient sera were also detected and their differing binding affinities determined. In general, when sera of normal subjects were found to contain free MBP-SFs, the reagent anti-MBP antibodies in the reagent antiserum used to detect them were of relatively high binding affinity (8 X 10(8) M-1). When normal sera were found to contain free anti-MBP antibodies, the affinities of such antibodies were invariably lower (0.06-0.7 X 10(8) M-1). In contrast, sera of patients with active MS and exhibiting clinical fluctuations in their disease, infrequently contained high or medium high affinity MBP-SFs, whereas higher affinity anti-MBP antibodies were commonly detected. These patterns of MBP-SFs and anti-MBP antibodies in normal and MS human sera resemble those previously observed in studies of normal Lewis rats and rats developing experimental allergic encephalomyelitis (EAE). The findings here reported provide additional support for the view that circulating endogenous MBP-SFs may function as neuroautotolerogens that restrict expansion of MBP-reactive lymphoid cell clones having potentially injurious effector activity for central nervous system (CNS) tissue.
正常人和多发性硬化症（MS）患者的血清中经常发现含有皮摩尔量的内源性髓鞘碱性蛋白-血清因子（MBP-SFs）。这些血清因子共同构成了一个异质谱，通过一种竞争性抑制放射免疫测定法（RIA）进行检测和测量，该方法旨在区分它们与抗MBP试剂抗血清的各自结合亲和力。这些正常和患者血清中的抗MBP抗体也被检测到，并确定了它们不同的结合亲和力。一般来说，当发现正常受试者的血清中含有游离的MBP-SFs时，用于检测它们的试剂抗血清中的试剂抗MBP抗体具有相对较高的结合亲和力（8×10⁸ M⁻¹）。当发现正常血清中含有游离的抗MBP抗体时，这些抗体的亲和力总是较低（0.06 - 0.7×10⁸ M⁻¹）。相比之下，患有活动性MS且疾病表现出临床波动的患者血清中，很少含有高亲和力或中等高亲和力的MBP-SFs，而通常能检测到较高亲和力的抗MBP抗体。正常人和MS患者血清中MBP-SFs和抗MBP抗体的这些模式与先前在正常Lewis大鼠和患实验性变应性脑脊髓炎（EAE）的大鼠研究中观察到的模式相似。此处报告的研究结果为以下观点提供了额外支持，即循环内源性MBP-SFs可能作为神经自身耐受原发挥作用，限制对中枢神经系统（CNS）组织具有潜在损伤效应活性的MBP反应性淋巴细胞克隆的扩增。
