人肾素cDNA片段的分子克隆及核苷酸序列

Soubrier F, Panthier J J, Corvol P, Rougeon F

Nucleic Acids Res. 1983 Oct 25;11(20):7181-90. doi: 10.1093/nar/11.20.7181.

We have studied human renin messenger RNA by hybridization with the mouse submaxillary gland (SMG) renin cDNA probe. The human kidney messenger RNA is about 1.6 kilobase (kb) long, similarly to the mouse SMG renin mRNA. A kidney renin cDNA clone of 1.1 kb length was obtained. A comparison of nucleotide sequences of mouse and human cDNA clones reveals conservation of residues involved in catalytic mechanisms and a potential glycosylation site. The human renin molecular probe allowed us to study renin expression in human chorionic tissue. The chorionic and kidney renin messenger RNAs are similar in length. The Southern blot analysis reveals the presence of a single renin gene in human DNA.

我们通过与小鼠颌下腺（SMG）肾素cDNA探针杂交，对人肾素信使RNA进行了研究。人肾脏信使RNA长度约为1.6千碱基（kb），与小鼠SMG肾素mRNA相似。获得了一个长度为1.1 kb的肾脏肾素cDNA克隆。对小鼠和人cDNA克隆的核苷酸序列比较显示，参与催化机制的残基以及一个潜在的糖基化位点具有保守性。人肾素分子探针使我们能够研究人绒毛膜组织中的肾素表达。绒毛膜和肾脏肾素信使RNA长度相似。Southern印迹分析显示人DNA中存在单个肾素基因。