甲病毒在病毒粒子和受感染细胞上的特异性和交叉反应性抗原的比较。
Comparison of specific and cross-reactive antigens of alphaviruses on virions and infected cells.
作者信息
Gates D, Brown A, Wust C J
出版信息
Infect Immun. 1982 Jan;35(1):248-55. doi: 10.1128/iai.35.1.248-255.1982.
Abstract
Rabbit hyperimmune antisera against Sindbis (SIN) or Semliki Forest (SF) virus were absorbed with purified SIN virus or SIN virus-infected cells, or with SF virus or SF virus-infected cells. Residual antibody titers were determined by hemagglutination inhibition (HAI) and antibody-dependent, complement-mediated cytolysis (ADCMC) assays. It appeared that absorption with virus-infected cells removed ADCMC-detectable cross-reactive antibody much more efficiently than did absorption with either virus. HAI assays with the same absorbed antisera indicated that both virus and virus-infected cells removed HAI-detectable cross-reactive antibody. On the basis of these and other data, there appeared to be a cross-reactive antigen present on virus-infected cells which was detectable by ADCMC and was distinct from the cross-reactive antigen assayed by HAI.
摘要
用纯化的辛德毕斯（SIN）病毒或感染SIN病毒的细胞，或用塞姆利基森林（SF）病毒或感染SF病毒的细胞吸收兔抗SIN或抗SF病毒的超免疫抗血清。通过血凝抑制（HAI）和抗体依赖性补体介导的细胞溶解（ADCMC）试验测定残留抗体滴度。结果表明，用感染病毒的细胞吸收比用任何一种病毒吸收更有效地去除了ADCMC可检测到的交叉反应性抗体。用相同吸收抗血清进行的HAI试验表明，病毒和感染病毒的细胞都去除了HAI可检测到的交叉反应性抗体。基于这些及其他数据，似乎在感染病毒的细胞上存在一种交叉反应性抗原，它可被ADCMC检测到，且与HAI检测的交叉反应性抗原不同。
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