Sefton B M, Burge B W
J Virol. 1973 Dec;12(6):1366-74. doi: 10.1128/JVI.12.6.1366-1374.1973.
The sequence in which sugars are added to the Sindbis virus glycoproteins was studied. Infected cells contain three glycosylated virus-specific proteins: the two virion glycoproteins and the immediate precursor to the smaller virion glycoprotein. Larger Sindbis-specific proteins are not glycosylated. The cell-associated forms of both of the virion glycoproteins contain glucosamine, mannose, galactose, and fucose. The glycosylated precursor contains only glucosamine, mannose, and some galactose. The conversion of precursor to virion protein involves both the addition of galactose and fucose and the loss of mannose. The apparent extent of glycosylation of each virus-specific protein is not influenced by the host cell.
研究了糖添加到辛德毕斯病毒糖蛋白中的顺序。受感染的细胞含有三种糖基化的病毒特异性蛋白：两种病毒粒子糖蛋白以及较小病毒粒子糖蛋白的直接前体。较大的辛德毕斯特异性蛋白未被糖基化。两种病毒粒子糖蛋白的细胞相关形式都含有葡糖胺、甘露糖、半乳糖和岩藻糖。糖基化前体仅含有葡糖胺、甘露糖和一些半乳糖。前体向病毒粒子蛋白的转化涉及半乳糖和岩藻糖的添加以及甘露糖的丢失。每种病毒特异性蛋白的糖基化明显程度不受宿主细胞的影响。
