Berglin E H, Edlund M B, Nyberg G K, Carlsson J
J Bacteriol. 1982 Oct;152(1):81-8. doi: 10.1128/jb.152.1.81-88.1982.
Under anaerobic conditions an exponentially growing culture of Escherichia coli K-12 was exposed to hydrogen peroxide in the presence of various compounds. Hydrogen peroxide (0.1 mM) together with 0.1 mM L-cysteine or L-cystine killed the organisms more rapidly than 10 mM hydrogen peroxide alone. The exposure of E. coli to hydrogen peroxide in the presence of L-cysteine inhibited some of the catalase. This inhibition, however, could not fully explain the 100-fold increase in hydrogen peroxide sensitivity of the organism in the presence of L-cysteine. Of other compounds tested only some thiols potentiated the bactericidal effect of hydrogen peroxide. These thiols were effective, however, only at concentrations significantly higher than 0.1 mM. The effect of L-cysteine and L-cystine could be annihilated by the metal ion chelating agent 2,2'-bipyridyl. DNA breakage in E. coli K-12 was demonstrated under conditions where the organisms were killed by hydrogen peroxide.
在厌氧条件下，将指数生长的大肠杆菌K-12培养物在各种化合物存在的情况下暴露于过氧化氢。过氧化氢（0.1 mM）与0.1 mM L-半胱氨酸或L-胱氨酸一起比单独使用10 mM过氧化氢更快地杀死细菌。在L-半胱氨酸存在的情况下，大肠杆菌暴露于过氧化氢会抑制一些过氧化氢酶。然而，这种抑制不能完全解释在L-半胱氨酸存在下该生物体对过氧化氢敏感性增加100倍的现象。在所测试的其他化合物中，只有一些硫醇增强了过氧化氢的杀菌作用。然而，这些硫醇只有在浓度明显高于0.1 mM时才有效。L-半胱氨酸和L-胱氨酸的作用可以被金属离子螯合剂2,2'-联吡啶消除。在细菌被过氧化氢杀死的条件下，证明了大肠杆菌K-12中的DNA断裂。
