Effect of methisoprinol on E-rosette formation by trypsin-treated lymphocytes.

Human lymphocytes lose the capacity to form E rosettes after trypsin treatment. This ability is partially restored, in normal conditions, by a prolonged incubation in fresh medium supplemented with FCS. By adding methisoprinol to trypsin-treated lymphocytes, it has been observed a significant recovery after 2 hours of incubation, without further enhancement up to 24 hours, when compared to the controls. These results imply that the drug effect is time-dependent and that methisoprinol acts likely by a metabolic pathway in the early resynthesis of E receptors on trypsin-treated lymphocytes.