Jefferis R, Lowe J, Ling N R, Porter P, Senior S
Immunology. 1982 Jan;45(1):71-7.
Antibody-producing hybridoma clones have been isolated following immunization of mice with human IgG. Twenty-five monoclonal antibodies (nine anti-C gamma 3, fourteen anti-C gamma 2, one anit-kappa and one anti-lambda) were selected for study of their cross-reactivity with the IgG of fifteen mammalian species and chicken immunoglobulin. Each antibody exhibited a unique reaction profile suggesting that human IgG expresses a very large repertoire of immunogenic epitopes. Whilst some antibodies showed a very restricted cross-reactivity profile for others a very wide reactivity profile was observed-including two clones producing autoantibodies. Antibodies demonstrating cross-reactivity between human Fc gamma and 7S chicken immunoglobulin allow its definitive assignment as a homologue of human IgG. Four clones demonstrated specificity for bovine IgG subclass gamma 1 and gamma 2 and the degree of reactivity allows their application to qualitative and quantitative assay systems. These studies suggest new perspectives for the characterization of immunoglobulins and the standardization of anti-immunoglobulin reagents.
用人IgG免疫小鼠后分离出了产生抗体的杂交瘤克隆。选择了25种单克隆抗体（9种抗Cγ3、14种抗Cγ2、1种抗κ和1种抗λ）来研究它们与15种哺乳动物和鸡免疫球蛋白的IgG的交叉反应性。每种抗体都表现出独特的反应模式，这表明人IgG表达了非常大量的免疫原性表位。虽然一些抗体显示出非常有限的交叉反应性模式，但其他抗体则观察到非常广泛的反应性模式，包括两个产生自身抗体的克隆。证明人Fcγ与7S鸡免疫球蛋白之间存在交叉反应性的抗体使其被明确认定为人IgG的同源物。四个克隆对牛IgG亚类γ1和γ2具有特异性，反应程度使其可应用于定性和定量分析系统。这些研究为免疫球蛋白的表征和抗免疫球蛋白试剂的标准化提供了新的视角。
