Tada N, Kimura S, Liu Y, Taylor B A, Hämmerling U
Immunogenetics. 1981;13(6):539-46. doi: 10.1007/BF00343721.
Spleen cells from an SJL mouse immunized with 70Z/3 cells, an established pre-B cell line, were fused with cells of the nonsecretory myeloma line NS.1. One established hybridoma cell line (clone K10.6) continuously secreted antibody that recognized a new antigenic specificity tentatively named Ly-m19. This newly found antigen is detectable on both T and B cells. Cytotoxicity assays reveal that 75 percent of the spleen and lymph-node cells, 35 percent of bone-marrow cells, and 15 percent of thymus cells reacted with antibody of clone K10.6. Strains expressing the specificity Ly-m19.1 are characterized by negative reactions and include the strains AKR, CE/J, RF/J, GR/A, SJL, P/J, BDP/J, and LG/J. All other strains so far tested are Ly-m19.2. This strain distribution pattern distinguishes Ly-m19 from any known murine lymphocyte alloantigen, but it parallels the Lyb-2c haplotype. Linkage test of a set of AKXL recombinant inbred strains revealed close linkage of Ly-m19 and Lyb-2 loci on mouse chromosome 4.
用已建立的前B细胞系70Z/3细胞免疫的SJL小鼠的脾细胞，与非分泌性骨髓瘤细胞系NS.1的细胞进行融合。一个已建立的杂交瘤细胞系（克隆K10.6）持续分泌识别一种新抗原特异性的抗体，该抗原暂命名为Ly-m19。这种新发现的抗原在T细胞和B细胞上均可检测到。细胞毒性试验显示，75%的脾细胞和淋巴结细胞、35%的骨髓细胞以及15%的胸腺细胞与克隆K10.6的抗体发生反应。表达特异性Ly-m19.1的品系表现为阴性反应，包括AKR、CE/J、RF/J、GR/A、SJL、P/J、BDP/J和LG/J品系。到目前为止测试的所有其他品系均为Ly-m19.2。这种品系分布模式使Ly-m19与任何已知的小鼠淋巴细胞同种异体抗原区分开来，但它与Lyb-2c单倍型相似。对一组AKXL重组近交系进行的连锁测试显示，Ly-m19和Lyb-2基因座在小鼠4号染色体上紧密连锁。
