Neville M E, Lischner H W
J Immunol. 1982 Mar;128(3):1063-9.
Activation of human peripheral blood lymphocytes by incubation with particulate immune complexes or aggregated human gamma-globulin was studied by measuring the release of leukocyte migration inhibitory factor (LIF) activity. LIF-active supernatants were consistently produced when nonadherent lymphocytes containing less than 1% surface immunoglobulin-bearing cells and less than 0.2% nonspecific esterase-positive monocytes were incubated in the presence of RBC sensitized with rabbit or human antibodies or with pooled heat-aggregated human gamma-globulin. This immune complex-induced lymphokine production (ICLP) was dependent on the presence of cells bearing receptors for the Fc portion of IgG (Fc gamma). ICLP could not be demonstrated with lymphocyte preparations enriched for B cells even though the latter showed vigorous LIF production in the presence of complement-sensitized erythrocytes. ICLP was dependent on the concentration of lymphocytes and of stimulant as well as on the duration of coincubation, and it required active metabolic processes and RNA and protein synthesis but not DNA synthesis. Ca++ but not Mg++ was obligatory. ICLP by non-B Fc gamma receptor-bearing lymphocytes may play a role in antibody-dependent protective inflammation and immunologic injury phenomena, which is similar to that of lymphokine release by antigen-activated T cells in delayed hypersensitivity responses.
通过测量白细胞迁移抑制因子（LIF）活性，研究了用颗粒状免疫复合物或聚合人γ球蛋白孵育人外周血淋巴细胞的激活情况。当含有少于1%表面带有免疫球蛋白细胞和少于0.2%非特异性酯酶阳性单核细胞的非贴壁淋巴细胞，在经兔或人抗体致敏的红细胞或混合热聚合人γ球蛋白存在的情况下孵育时，始终会产生具有LIF活性的上清液。这种免疫复合物诱导的淋巴因子产生（ICLP）依赖于带有IgG Fc部分受体（Fcγ）的细胞的存在。即使富含B细胞的淋巴细胞制剂在补体致敏红细胞存在的情况下显示出旺盛的LIF产生，但用其却无法证明ICLP。ICLP依赖于淋巴细胞和刺激物的浓度以及共孵育的持续时间，并且它需要活跃的代谢过程以及RNA和蛋白质合成，但不需要DNA合成。Ca++是必需的，而Mg++不是。带有非B Fcγ受体的淋巴细胞产生的ICLP可能在抗体依赖性保护性炎症和免疫损伤现象中起作用，这类似于抗原激活的T细胞在迟发型超敏反应中释放淋巴因子的作用。
