Wang B S, Heacock E H, Chang-Xue Z, Tilney N L, Strom T B, Mannick J A
J Immunol. 1982 Mar;128(3):1382-5.
Mice sensitized with alloantigens and treated with cyclosporin A (CsA) were incapable of generating antigen-specific cytolytic lymphocytes (CL). Lymphocytes from these CsA-treated animals could not be reactivated upon exposure to the same alloantigens in mixed lymphocyte culture (MLC), whereas their response to a third-party antigen remained intact, suggesting a long-lasting and specific effect of CsA. After being irradiated, these lymphocytes from CsA-treated animals were added to normal MLC and were shown to prevent normal lymphocytes from becoming cytolytic in a dose-dependent and antigen-nonspecific fashion. These suppressor cells were not detected in mice receiving CsA only, indicating that CsA did not induce but rather permitted the expression of suppressor cells possibly generated by allosensitization. The suppressor cells appeared to be T lymphocytes, because treatment with anti-Thy-1.2 antibody and C abrogated their suppressive activity. The present results suggest that activation and/or sparing of suppressor cells by CsA may account for the long-lasting unresponsiveness seen in CsA-treated animals.
用同种异体抗原致敏并用环孢素A（CsA）处理的小鼠无法产生抗原特异性细胞溶解淋巴细胞（CL）。来自这些经CsA处理动物的淋巴细胞在混合淋巴细胞培养（MLC）中暴露于相同的同种异体抗原时不能被重新激活，而它们对第三方抗原的反应保持完整，这表明CsA具有持久且特异性的作用。经照射后，将这些来自经CsA处理动物的淋巴细胞添加到正常的MLC中，结果显示它们能以剂量依赖性和抗原非特异性的方式阻止正常淋巴细胞变成细胞溶解状态。在仅接受CsA的小鼠中未检测到这些抑制细胞，这表明CsA不是诱导而是允许可能由同种异体致敏产生的抑制细胞的表达。这些抑制细胞似乎是T淋巴细胞，因为用抗Thy-1.2抗体和补体处理可消除它们的抑制活性。目前的结果表明，CsA对抑制细胞的激活和/或保留可能是经CsA处理的动物中出现持久无反应性的原因。
