Wang B S, Heacock E H, Collins K H, Hutchinson I F, Tilney N L, Mannick J A
J Immunol. 1981 Jul;127(1):89-93.
The purpose of the present study was the investigation of the effect of cyclosporin A (CsA) on the induction of alloreactivity in vitro and in vivo. Addition of CsA to mouse mixed lymphocyte cultures (MLC) not only inhibited lymphocyte proliferation but also prevented the generation of alloreactive cytolytic lymphocytes (CL). It was necessary to add CsA within the first 3 days of a 5-day MLC in order to achieve a significant suppressive effect. Lymphocytes, after being cultured in MLC with CsA for 4 days or longer, were incapable of being activated upon re-exposure to the same alloantigens although their responses to unrelated antigens remained intact, indicating antigen specificity of the suppression induced by CsA and its long-lasting effect. Furthermore, lymphocytes from mice treated with CsA after allosensitization failed to manifest primary cytotoxicity and could not be reactivated in a secondary MLC. Finally, CsA had no effect on those CL already generated, suggesting that CsA acts upon the induction of CL rather than the effector phase.
本研究的目的是调查环孢素A（CsA）在体外和体内对同种异体反应性诱导的影响。将CsA添加到小鼠混合淋巴细胞培养物（MLC）中，不仅抑制淋巴细胞增殖，还能阻止同种异体反应性细胞溶解淋巴细胞（CL）的产生。为了达到显著的抑制效果，必须在为期5天的MLC的前3天内添加CsA。淋巴细胞在含有CsA的MLC中培养4天或更长时间后，再次接触相同的同种异体抗原时无法被激活，尽管它们对无关抗原的反应保持完整，这表明CsA诱导的抑制具有抗原特异性及其持久效应。此外，同种异体致敏后用CsA处理的小鼠的淋巴细胞未能表现出初次细胞毒性，并且在二次MLC中无法被重新激活。最后，CsA对已经产生的那些CL没有影响，这表明CsA作用于CL的诱导而非效应阶段。
