Uchida A, Micksche M
Int J Immunopharmacol. 1981;3(4):365-75. doi: 10.1016/0192-0561(81)90032-1.
OK-432, a streptococcal preparation, augmented the natural killing (NK) activity of peripheral blood lymphocytes of normal donors and cancer patients against both NK sensitive and resistant human target cells in vitro. The enhancement of NK activity was evident after 4 h pretreatment and maximum by 16-24 h. The manifestation of OK-432 induced augmentation required active cell metabolism, RNA and protein synthesis but no DNA synthesis of lymphocytes. The supernatants produced by OK-432 stimulated lymphocyte cultures had no enhancing substance nor interferon. Anti-interferon antibodies did not inhibit boosting activity of OK-432. Large granular lymphocytes were involved in both spontaneous and OK-432 induced cytotoxic activity. The proportion of lymphocytes conjugating to target cells was not changed by OK-432. These results suggest that OK-432 augments cytotoxic activity of large granular lymphocytes having ability to recognize target cells independent of interferon induction.
链球菌制剂OK-432在体外增强了正常供体和癌症患者外周血淋巴细胞对NK敏感和抗性人类靶细胞的自然杀伤（NK）活性。NK活性在预处理4小时后明显增强，在16 - 24小时达到最大值。OK-432诱导的增强作用的表现需要淋巴细胞的活跃细胞代谢、RNA和蛋白质合成，但不需要DNA合成。OK-432刺激的淋巴细胞培养物产生的上清液没有增强物质也没有干扰素。抗干扰素抗体不抑制OK-432的增强活性。大颗粒淋巴细胞参与了自发的和OK-432诱导的细胞毒性活性。OK-432没有改变与靶细胞结合的淋巴细胞比例。这些结果表明，OK-432增强了具有识别靶细胞能力的大颗粒淋巴细胞的细胞毒性活性，且不依赖于干扰素诱导。
