Kruh J
Mol Cell Biochem. 1982 Feb 5;42(2):65-82. doi: 10.1007/BF00222695.
Sodium butyrate, at millimolar concentrations, when added to cell cultures produces many morphological and biochemical modifications in a reversible manner. Some of them occur in all cell lines. They concern regulatory mechanisms of gene expression and cell growth: an hyperacetylation of histone resulting from an inhibition of histone deacetylase and an arrest of cell proliferation are almost constantly observed. Some other modifications vary from one cell type to another: induction of proteins, including enzymes, hormones, hemoglobin, inhibition of cell differentiation, reversion of transformed characteristics of cells to normal morphological and biochemical pattern, increase in interferon antiviral efficiency and induction of integrated viruses. Most if not all these effects of butyrate could result from histone hyperacetylation, from changes in chromatin structures as measured by accessibility to DNases and from modifications in cytoskeleton assembly. We do not know at the present time whether butyrate acts on a very specific target site in cell or if it acts on several cell components.
毫摩尔浓度的丁酸钠添加到细胞培养物中时，会以可逆方式产生许多形态学和生物化学变化。其中一些变化在所有细胞系中都会出现。它们涉及基因表达和细胞生长的调控机制：几乎总能观察到由于组蛋白脱乙酰酶受抑制导致的组蛋白高度乙酰化以及细胞增殖停滞。其他一些变化则因细胞类型而异：诱导包括酶、激素、血红蛋白在内的蛋白质，抑制细胞分化，使细胞的转化特征恢复到正常的形态学和生物化学模式，提高干扰素抗病毒效率以及诱导整合病毒。丁酸盐的这些作用，即便不是全部，大多数可能是由组蛋白高度乙酰化、通过对脱氧核糖核酸酶的可及性测量得出的染色质结构变化以及细胞骨架组装的改变所导致的。目前我们尚不清楚丁酸盐是作用于细胞中一个非常特定的靶点，还是作用于多个细胞成分。
