Hogarth P M, Edwards J, McKenzie I F, Goding J W, Liew F Y
Immunology. 1982 May;46(1):135-44.
Six monoclonal anti-Ly-2.1 antibodies are described that arose from a single fusion using the spleen from a 129/ReJ mouse immunized with CBA/H thymus cells. The specificity was determined from the strain distribution, and testing of congenic strains where all six monoclonal antibodies detected the Ly-2.1 alloantigen. Furthermore, the antibodies fall into two groups: Group I (four antibodies) detected the expected number of Ly-2+ thymocytes and peripheral T cells, whereas Group II (two antibodies) detected 10% fewer peripheral T cells. Functional studies demonstrated that the Ly-2.1 determinant detected by a Group I antibody (49-31.1) was presented on cytotoxic T cells (Ly-1+2+), on concanavalin A (Con A)-induced suppressor T cells (Ly-1-2+), but absent from helper T cells (Ly-1+2-). One antibody (49-11.1) precipitated a 68,000-75,000 mol.wt glycoprotein, which on reduction yielded 30,000 and 35,000 mol.wt moieties. Thus, by functional, genetic and biochemical criteria these antibodies detected the Ly-2.1 specificity. In addition, a monoclonal, noncytotoxic, IgGl, anti-Thy-1.2 antibody is described.
描述了六种抗Ly-2.1单克隆抗体，它们源自用CBA/H胸腺细胞免疫的129/ReJ小鼠脾脏进行的一次融合。通过品系分布以及对同基因品系的检测确定了特异性，在这些同基因品系中，所有六种单克隆抗体均能检测到Ly-2.1同种异体抗原。此外，这些抗体分为两组：第一组（四种抗体）检测到预期数量的Ly-2+胸腺细胞和外周T细胞，而第二组（两种抗体）检测到的外周T细胞数量少10%。功能研究表明，第一组抗体（49-31.1）检测到的Ly-2.1决定簇存在于细胞毒性T细胞（Ly-1+2+）、刀豆蛋白A（Con A）诱导的抑制性T细胞（Ly-1-2+）上，但在辅助性T细胞（Ly-1+2-）上不存在。一种抗体（49-11.1）沉淀出一种分子量为68,000 - 75,000的糖蛋白，还原后产生分子量为30,000和35,000的部分。因此，根据功能、遗传和生化标准，这些抗体检测到了Ly-2.1特异性。此外，还描述了一种单克隆、无细胞毒性的IgG1抗Thy-1.2抗体。
