Neimark H, London J
J Bacteriol. 1982 Jun;150(3):1259-65. doi: 10.1128/jb.150.3.1259-1265.1982.
We report the establishment of a phylogenetic relationship between the sterol-nonrequiring mycoplasmas (Acholeplasma species) and streptococci. Three specific antisera prepared against purified Streptococcus faecalis fructose diphosphate aldolase and glyceraldehyde-3-phosphate dehydrogenase and Pediococcus cerevisiae glyceraldehyde-3-phosphate dehydrogenase were used for comparative enzyme immunological studies; the Ouchterlony double-diffusion technique and the quantitative microcomplement fixation procedure were employed. The reactions obtained provide evidence showing that all seven ACholeplasma species studied (A. laidlawii, A. granularum, A. modicum, A. oculi, A. axanthum. A. hippikon, and A. equifetale) are phylogenetically related to streptococci and that they evolved from streptococci. The data strongly suggest that the acholeplasmas comprise a distinct evolutionary group that has diverged from streptococci belonging to Lancefield group D or N. No reactions were observed between these enzyme antisera and cell extracts from six fermentative Mycoplasma species. These results support the view that mycoplasmas are derived from various bacteria.
我们报告了无甾醇支原体（无胆甾原体属菌种）与链球菌之间系统发育关系的确立。针对纯化的粪肠球菌果糖二磷酸醛缩酶、3-磷酸甘油醛脱氢酶以及酿酒片球菌3-磷酸甘油醛脱氢酶制备的三种特异性抗血清，用于比较酶免疫研究；采用了欧氏双扩散技术和定量微量补体结合程序。所获得的反应结果表明，所研究的所有七种无胆甾原体属菌种（莱氏无胆甾原体、颗粒无胆甾原体、适度无胆甾原体、眼无胆甾原体、黄无胆甾原体、马无胆甾原体和马胎儿无胆甾原体）在系统发育上与链球菌相关，且它们是从链球菌进化而来的。数据有力地表明，无胆甾原体构成了一个与属于兰斯菲尔德D组或N组的链球菌不同的进化群体。在这些酶抗血清与六种发酵支原体菌种的细胞提取物之间未观察到反应。这些结果支持了支原体源自各种细菌的观点。
