Lesot H, Meyer J M, Ruch J V, Weber K, Osborn M
Differentiation. 1982;21(2):133-7. doi: 10.1111/j.1432-0436.1982.tb01206.x.
The localization of constitutive proteins of different types of cytoskeletal components (prekeratin, vimentin, and actin) was examined in embryonic mouse molars using specific antibodies and immunofluorescence microscopy on frozen sections. Prekeratin and actin were found in the enamel organ. Preameloblasts demonstrated uniform staining, whereas ameloblasts demonstrated an apical accumulation of both prekeratin and actin. Vimentin and actin were observed in the dental papilla. A redistribution of vimentin accompanied the polarization of odontoblasts. A possible transmembranous control of cytoskeletal activities by the extracellular matrix is discussed.
利用特异性抗体和冷冻切片免疫荧光显微镜技术，在胚胎小鼠磨牙中检测了不同类型细胞骨架成分（前角蛋白、波形蛋白和肌动蛋白）组成蛋白的定位。在前成釉细胞中前角蛋白和肌动蛋白呈均匀染色，而成釉细胞中前角蛋白和肌动蛋白均在顶端聚集。在牙乳头中观察到波形蛋白和肌动蛋白。波形蛋白的重新分布伴随着成牙本质细胞的极化。本文还讨论了细胞外基质对细胞骨架活动可能存在的跨膜调控。
