Altmannsberger M, Weber K, Hölscher A, Schauer A, Osborn M
Lab Invest. 1982 May;46(5):520-6.
Twenty-five primary gastrointestinal carcinomas have been studied using immunofluorescence microscopy with affinity-purified antibodies to prekeratin and to vimentin. The tissues were alcohol fixed and paraffin embedded before use. In all cases (i.e., one case of esophagal carcinoma, seven stomach carcinomas, and 17 large bowel carcinomas) the tumor cells are stained by antibodies to prekeratin. In cases in which only very few tumor cells are present, such as signet ring carcinoma, immunofluorescence with prekeratin antibody provides an easy way to visualize single tumor cells. When the same specimens were tested with antibodies to vimentin, the tumor cells were unstained, and only the fibroblasts and vessels of the stroma were decorated. Four of the tumors were also negative when tested with antibodies specific for either desmin, or glial fibrillary acidic protein or neurofilaments. Three metastases to the abdominal region from tumors originating in the ovary, stomach, and large bowel were like the primary tumors in that the tumor cells were positive when stained with antibodies to prekeratin and negative when tested with the antibodies to vimentin.
使用针对前角蛋白和波形蛋白的亲和纯化抗体，通过免疫荧光显微镜对25例原发性胃肠道癌进行了研究。这些组织在使用前用酒精固定并石蜡包埋。在所有病例中（即1例食管癌、7例胃癌和17例大肠癌），肿瘤细胞均被前角蛋白抗体染色。在肿瘤细胞极少的病例中，如印戒细胞癌，前角蛋白抗体免疫荧光为观察单个肿瘤细胞提供了一种简便方法。当用波形蛋白抗体检测相同标本时，肿瘤细胞未被染色，仅基质中的成纤维细胞和血管被标记。当用结蛋白、胶质纤维酸性蛋白或神经丝特异性抗体检测时，其中4例肿瘤也呈阴性。来自卵巢、胃和大肠原发肿瘤的3例腹部转移瘤与原发肿瘤相似，即肿瘤细胞用前角蛋白抗体染色时呈阳性，用波形蛋白抗体检测时呈阴性。
