一种用于染色鹦鹉热衣原体和沙眼衣原体包涵体的快速方法。
A rapid method for staining inclusions of Chlamydia psittaci and Chlamydia trachomatis.
作者信息
Woodland R M, Malam J, Darougar S
出版信息
J Clin Pathol. 1982 Jun;35(6):642-4. doi: 10.1136/jcp.35.6.642.
Abstract
A new staining method was developed for the detection of inclusions of Chlamydia psittaci and Chlamydia trachomatis inclusions in cell cultures. Using a combination of methyl green and neutral red stains and washing at pH 5.0, inclusions were stained red while cell cytoplasm was pale pink and cell nuclei were pale green. The method was significantly better than Giemsa staining and comparable to immunofluorescence for detecting C psittaci inclusions. Its sensitivity for detection C trachomatis inclusions by dark field microscopy was similar to that of Giemsa staining.
摘要
开发了一种新的染色方法，用于检测细胞培养物中鹦鹉热衣原体和沙眼衣原体的包涵体。使用甲基绿和中性红染色剂组合，并在pH 5.0下洗涤，包涵体被染成红色，而细胞质呈淡粉色，细胞核呈淡绿色。该方法在检测鹦鹉热衣原体包涵体方面明显优于吉姆萨染色，与免疫荧光法相当。其通过暗视野显微镜检测沙眼衣原体包涵体的灵敏度与吉姆萨染色相似。
相似文献
1
A rapid method for staining inclusions of Chlamydia psittaci and Chlamydia trachomatis.一种用于染色鹦鹉热衣原体和沙眼衣原体包涵体的快速方法。
J Clin Pathol. 1982 Jun;35(6):642-4. doi: 10.1136/jcp.35.6.642.
2
Detection of chlamydial inclusions in cell culture or biopsy tissue by alkaline phosphatase-anti-alkaline phosphatase staining.通过碱性磷酸酶-抗碱性磷酸酶染色检测细胞培养物或活检组织中的衣原体包涵体。
J Clin Microbiol. 1987 Oct;25(10):1864-7. doi: 10.1128/jcm.25.10.1864-1867.1987.
3
Detection of Chlamydia trachomatis inclusions in McCoy and HeLa-229 cells: an alternative staining technique using toluidine blue.在 McCoy 细胞和 HeLa - 229 细胞中检测沙眼衣原体包涵体：一种使用甲苯胺蓝的替代染色技术。
J Clin Pathol. 1984 Jun;37(6):682-5. doi: 10.1136/jcp.37.6.682.
4
Sensitivity of immunoperoxidase and immunofluorescence staining for detecting chlamydia in conjunctival scrapings and in cell culture.免疫过氧化物酶和免疫荧光染色法检测结膜刮片及细胞培养中衣原体的敏感性。
J Clin Pathol. 1978 Nov;31(11):1073-7. doi: 10.1136/jcp.31.11.1073.
5
Sensitivity of immunofluorescence with monoclonal antibodies for detection of Chlamydia trachomatis inclusions in cell culture.用单克隆抗体进行免疫荧光检测细胞培养中沙眼衣原体包涵体的敏感性。
J Clin Microbiol. 1982 Jul;16(1):4-7. doi: 10.1128/jcm.16.1.4-7.1982.
6
[Detection of chlamydia in patients with trachoma].[沙眼患者中衣原体的检测]
Cesk Oftalmol. 1985 Jul;41(4):209-15.
7
Detection of Chlamydia trachomatis inclusions in Mccoy cell cultures with fluorescein-conjugated monoclonal antibodies.使用荧光素偶联单克隆抗体检测 McCoy 细胞培养物中的沙眼衣原体包涵体。
J Clin Microbiol. 1983 Apr;17(4):666-8. doi: 10.1128/jcm.17.4.666-668.1983.
8
[Immunohistochemical determination of Chlamydia psittaci/pecorum and C.trachomatis in the piglet gut].[仔猪肠道中鹦鹉热衣原体/鸽衣原体和沙眼衣原体的免疫组织化学测定]
Zentralbl Veterinarmed B. 1995 Jul;42(5):266-76.
9
Prevalence of Chlamydia psittaci and Other Chlamydia Species in Wild Birds in Poland.波兰野生鸟类中鹦鹉热衣原体及其他衣原体种类的流行情况。
Vector Borne Zoonotic Dis. 2015 Nov;15(11):652-5. doi: 10.1089/vbz.2015.1814. Epub 2015 Oct 26.
10
Culture and isolation of Chlamydia trachomatis.
Methods Enzymol. 1994;236:377-90. doi: 10.1016/0076-6879(94)36028-6.
引用本文的文献
1
Detection of Chlamydia trachomatis inclusions in McCoy and HeLa-229 cells: an alternative staining technique using toluidine blue.在 McCoy 细胞和 HeLa - 229 细胞中检测沙眼衣原体包涵体：一种使用甲苯胺蓝的替代染色技术。
J Clin Pathol. 1984 Jun;37(6):682-5. doi: 10.1136/jcp.37.6.682.
本文引用的文献
1
A simple method for total particle counts of trachoma and inclusion blennorrhoea viruses.一种用于沙眼和包涵体性脓漏眼病毒总颗粒计数的简单方法。
Nature. 1962 Sep 1;195:923-4. doi: 10.1038/195923a0.
2
OCCURENCE OF GLYCOGEN IN INCLUSIONS OF THE PSITTACOSIS-LYMPHOGRANULOMA VENEREUM-TRACHOMA AGENTS.鹦鹉热-性病性淋巴肉芽肿-沙眼病原体包涵体中糖原的出现情况。
J Infect Dis. 1965 Apr;115:186-96. doi: 10.1093/infdis/115.2.186.
3
Chlamydial infection. Results of micro-immunofluorescence tests for the detection of type-specific antibody in certain chlamydial infections.衣原体感染。用于检测某些衣原体感染中型特异性抗体的微量免疫荧光试验结果。
Br J Vener Dis. 1972 Dec;48(6):452-9. doi: 10.1136/sti.48.6.452.
4
An improved method for demonstrating the growth of Chlamydiae in tissue culture.一种用于在组织培养中证明衣原体生长的改良方法。
Med Lab Sci. 1978 Jan;35(1):67-74.
5
Sensitivity of immunoperoxidase and immunofluorescence staining for detecting chlamydia in conjunctival scrapings and in cell culture.免疫过氧化物酶和免疫荧光染色法检测结膜刮片及细胞培养中衣原体的敏感性。
J Clin Pathol. 1978 Nov;31(11):1073-7. doi: 10.1136/jcp.31.11.1073.
6
A comparison of staining techniques for demonstrating group A chlamydia in tissue culture.用于在组织培养中显示A组衣原体的染色技术比较。
Med Lab Technol. 1975 Jul;32(3):233-8.
Zotero 插件