一种用于染色鹦鹉热衣原体和沙眼衣原体包涵体的快速方法。
A rapid method for staining inclusions of Chlamydia psittaci and Chlamydia trachomatis.
作者信息
Woodland R M, Malam J, Darougar S
出版信息
J Clin Pathol. 1982 Jun;35(6):642-4. doi: 10.1136/jcp.35.6.642.
A new staining method was developed for the detection of inclusions of Chlamydia psittaci and Chlamydia trachomatis inclusions in cell cultures. Using a combination of methyl green and neutral red stains and washing at pH 5.0, inclusions were stained red while cell cytoplasm was pale pink and cell nuclei were pale green. The method was significantly better than Giemsa staining and comparable to immunofluorescence for detecting C psittaci inclusions. Its sensitivity for detection C trachomatis inclusions by dark field microscopy was similar to that of Giemsa staining.
开发了一种新的染色方法，用于检测细胞培养物中鹦鹉热衣原体和沙眼衣原体的包涵体。使用甲基绿和中性红染色剂组合，并在pH 5.0下洗涤，包涵体被染成红色，而细胞质呈淡粉色，细胞核呈淡绿色。该方法在检测鹦鹉热衣原体包涵体方面明显优于吉姆萨染色，与免疫荧光法相当。其通过暗视野显微镜检测沙眼衣原体包涵体的灵敏度与吉姆萨染色相似。
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