Stephens R S, Kuo C C, Tam M R
J Clin Microbiol. 1982 Jul;16(1):4-7. doi: 10.1128/jcm.16.1.4-7.1982.
Monoclonal antibodies which recognize the species-specific major outer membrane protein antigen of Chlamydia trachomatis were used for immunofluorescence staining of chlamydial inclusions in cell culture. A total of 115 clinical specimens were inoculated onto replicate HeLa 229 cell monolayers and assayed for chlamydial inclusions by immunofluorescence staining and Giemsa staining. Of the isolates, 38 were detected by immunofluorescence staining on passage 1 and 1 was detected on passage 2; 23 isolates on passage 1 and 13 isolates on passage 2 were detected by Giemsa staining. Immunofluorescence staining was significantly more sensitive than Giemsa staining for detecting chlamydial inclusions, particularly from specimens containing low titers of Chlamydia.
识别沙眼衣原体种特异性主要外膜蛋白抗原的单克隆抗体被用于细胞培养中衣原体包涵体的免疫荧光染色。总共115份临床标本接种到重复的HeLa 229细胞单层上，并通过免疫荧光染色和吉姆萨染色检测衣原体包涵体。在分离株中，38株在传代1时通过免疫荧光染色检测到，1株在传代2时检测到；吉姆萨染色在传代1时检测到23株分离株，在传代2时检测到13株分离株。免疫荧光染色在检测衣原体包涵体方面比吉姆萨染色显著更敏感，尤其是对于含有低滴度衣原体的标本。
