用单克隆抗体进行免疫荧光检测细胞培养中沙眼衣原体包涵体的敏感性。
Sensitivity of immunofluorescence with monoclonal antibodies for detection of Chlamydia trachomatis inclusions in cell culture.
作者信息
Stephens R S, Kuo C C, Tam M R
出版信息
J Clin Microbiol. 1982 Jul;16(1):4-7. doi: 10.1128/jcm.16.1.4-7.1982.
Abstract
Monoclonal antibodies which recognize the species-specific major outer membrane protein antigen of Chlamydia trachomatis were used for immunofluorescence staining of chlamydial inclusions in cell culture. A total of 115 clinical specimens were inoculated onto replicate HeLa 229 cell monolayers and assayed for chlamydial inclusions by immunofluorescence staining and Giemsa staining. Of the isolates, 38 were detected by immunofluorescence staining on passage 1 and 1 was detected on passage 2; 23 isolates on passage 1 and 13 isolates on passage 2 were detected by Giemsa staining. Immunofluorescence staining was significantly more sensitive than Giemsa staining for detecting chlamydial inclusions, particularly from specimens containing low titers of Chlamydia.
摘要
识别沙眼衣原体种特异性主要外膜蛋白抗原的单克隆抗体被用于细胞培养中衣原体包涵体的免疫荧光染色。总共115份临床标本接种到重复的HeLa 229细胞单层上，并通过免疫荧光染色和吉姆萨染色检测衣原体包涵体。在分离株中，38株在传代1时通过免疫荧光染色检测到，1株在传代2时检测到；吉姆萨染色在传代1时检测到23株分离株，在传代2时检测到13株分离株。免疫荧光染色在检测衣原体包涵体方面比吉姆萨染色显著更敏感，尤其是对于含有低滴度衣原体的标本。
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本文引用的文献
1
TRIC AGENTS ISOLATED IN THE UNITED STATES. X. IMMUNOFLUORESCENCE IN THE DIAGNOSIS OF TRIC AGENT INFECTION IN MAN.在美国分离出的沙眼衣原体。十、免疫荧光法在人类沙眼衣原体感染诊断中的应用。
Proc Soc Exp Biol Med. 1965 Jul;119:722-8. doi: 10.3181/00379727-119-30282.
2
Correlation between morphological and biochemical changes and the appearance of infectivity in FL cell cultures infected with trachoma agent.感染沙眼病原体的FL细胞培养物中形态学和生化变化与感染性出现之间的相关性。
Ann N Y Acad Sci. 1962 Mar 5;98:62-81. doi: 10.1111/j.1749-6632.1962.tb30532.x.
3
The titration of trachoma and inclusion blennorrhoea viruses in cell cultures.沙眼病毒和包涵体性脓漏眼病毒在细胞培养中的滴定
J Gen Microbiol. 1960 Dec;23:613-9. doi: 10.1099/00221287-23-3-613.
4
Monoclonal antibodies to Chlamydia trachomatis: antibody specificities and antigen characterization.抗沙眼衣原体单克隆抗体：抗体特异性及抗原特性
J Immunol. 1982 Mar;128(3):1083-9.
5
Further classification of TRIC agents from ocular trachoma and other sources by the mouse toxicity prevention test.通过小鼠毒性预防试验对来自眼沙眼和其他来源的TRIC制剂进行进一步分类。
Am J Ophthalmol. 1967 May;63(5):Suppl:1469-78. doi: 10.1016/0002-9394(67)94133-5.
6
Primary isolation of TRIC organisms in HeLa 229 cells treated with DEAE-dextran.在用二乙氨基乙基葡聚糖处理的HeLa 229细胞中对沙眼衣原体相关微生物进行初次分离。
J Infect Dis. 1972 Jun;125(6):665-8. doi: 10.1093/infdis/125.6.665.
7
Conjugation of antibodies with fluorochromes: modifications to the standard methods.抗体与荧光染料的偶联：对标准方法的改进
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8
Cultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells.沙眼衣原体在经放线菌酮处理的 McCoy 细胞中的培养。
J Clin Microbiol. 1977 Oct;6(4):328-31. doi: 10.1128/jcm.6.4.328-331.1977.
9
Sensitivity of immunoperoxidase and immunofluorescence staining for detecting chlamydia in conjunctival scrapings and in cell culture.免疫过氧化物酶和免疫荧光染色法检测结膜刮片及细胞培养中衣原体的敏感性。
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10
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