使用荧光素偶联单克隆抗体检测 McCoy 细胞培养物中的沙眼衣原体包涵体。
Detection of Chlamydia trachomatis inclusions in Mccoy cell cultures with fluorescein-conjugated monoclonal antibodies.
作者信息
Stamm W E, Tam M, Koester M, Cles L
出版信息
J Clin Microbiol. 1983 Apr;17(4):666-8. doi: 10.1128/jcm.17.4.666-668.1983.
Abstract
We compared two methods for identification of Chlamydia trachomatis inclusions in McCoy cell monolayers: conventional iodine staining and immunofluorescence staining with monoclonal antibodies against the species-specific major outer membrane protein antigen of C. trachomatis. Among 878 urethral and cervical specimens tested in parallel, the immunofluorescence method detected eightfold more inclusions per monolayer, identified a higher proportion of positive specimens on first passage (98 versus 62% by iodine staining; P less than 0.01), and improved overall sensitivity (98% of total positive specimens detected versus 84% by iodine staining; P less than 0.01). Improved sensitivity was most evident in specimens with low numbers of inclusions. Compared with conventional iodine staining, immunofluorescence staining with monoclonal antibodies improves sensitivity and offers more rapid detection of chlamydial inclusions in cell culture.
摘要
我们比较了两种在 McCoy 细胞单层中鉴定沙眼衣原体包涵体的方法：传统碘染色法和使用抗沙眼衣原体种特异性主要外膜蛋白抗原的单克隆抗体进行免疫荧光染色法。在 878 份同时检测的尿道和宫颈标本中，免疫荧光法检测到每个单层中的包涵体数量是碘染色法的八倍，在首次传代时鉴定出的阳性标本比例更高（碘染色法为 62%，免疫荧光法为 98%；P<0.01），并且提高了总体灵敏度（免疫荧光法检测到的总阳性标本为 98%，碘染色法为 84%；P<0.01）。在包涵体数量较少的标本中，灵敏度的提高最为明显。与传统碘染色法相比，使用单克隆抗体的免疫荧光染色提高了灵敏度，并能在细胞培养中更快速地检测到衣原体包涵体。
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