石榴菌素与核酸及丙酮酸脱羧酶的相互作用。

The interaction of granaticin with nucleic acids and pyruvate decarboxylase.

作者信息

Gibson-Clay G, Byrn S R, Heinstein P

出版信息

J Pharm Sci. 1982 Apr;71(4):467-8. doi: 10.1002/jps.2600710427.

Abstract

The interaction of granaticin with two different yeast ribonucleic acids, dialyzed transfer RNA and calf thymus DNA was studied. Contrary to previous reports, no binding of granaticin to DNA or RNA was observed. The visible spectrum of granaticin on direct mixing or equilibrium dialysis of granaticin with RNA or DNA was unchanged. Furthermore, granaticin did not displace acridine orange from DNA in competitive binding studies using fluorescence polarization. However, granaticin was shown to inhibit pyruvate decarboxylase. From the Ki for granaticin (3.8 mM) it was concluded that granaticin is as efficient as other 1,4-naphthoquinones in inhibiting pyruvate decarboxylase.

摘要

研究了石榴菌素与两种不同的酵母核糖核酸（透析后的转移核糖核酸）以及小牛胸腺脱氧核糖核酸之间的相互作用。与之前的报道相反，未观察到石榴菌素与脱氧核糖核酸或核糖核酸有结合现象。在将石榴菌素与核糖核酸或脱氧核糖核酸直接混合或进行平衡透析时，石榴菌素的可见光谱未发生变化。此外，在使用荧光偏振的竞争性结合研究中，石榴菌素并未从脱氧核糖核酸中取代吖啶橙。然而，已证明石榴菌素可抑制丙酮酸脱羧酶。根据石榴菌素的抑制常数（3.8 mM）得出结论，石榴菌素在抑制丙酮酸脱羧酶方面与其他1,4 - 萘醌一样有效。